Marshall P N, Bentley S A, Lewis S M
J Clin Pathol. 1975 Aug;28(8):680-5. doi: 10.1136/jcp.28.8.680.
The staining properties of 43 commerical Romanowsky-type stains have been studied. Considerable differences in the appearance of stained blood films were observed with different batches of these stains, the staining of red cells being particularly variable. Attempts have been made to correlate staining patterns with stain composition as revealed by thin-layer chromatography and sulphated ash analyses. In this way it has been possible to define some essential requirements for satisfactory staining.
对43种市售罗曼诺夫斯基型染色剂的染色特性进行了研究。使用不同批次的这些染色剂时,观察到染色血涂片的外观存在显著差异,其中红细胞的染色变化尤为明显。已尝试将染色模式与通过薄层色谱法和硫酸灰分分析揭示的染色剂成分相关联。通过这种方式,已能够确定获得满意染色效果的一些基本要求。