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一些商用罗曼诺夫斯基染色剂的评估

An evaluation of some commerical Romanowsky stains.

作者信息

Marshall P N, Bentley S A, Lewis S M

出版信息

J Clin Pathol. 1975 Aug;28(8):680-5. doi: 10.1136/jcp.28.8.680.

DOI:10.1136/jcp.28.8.680
PMID:52658
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC475801/
Abstract

The staining properties of 43 commerical Romanowsky-type stains have been studied. Considerable differences in the appearance of stained blood films were observed with different batches of these stains, the staining of red cells being particularly variable. Attempts have been made to correlate staining patterns with stain composition as revealed by thin-layer chromatography and sulphated ash analyses. In this way it has been possible to define some essential requirements for satisfactory staining.

摘要

对43种市售罗曼诺夫斯基型染色剂的染色特性进行了研究。使用不同批次的这些染色剂时,观察到染色血涂片的外观存在显著差异,其中红细胞的染色变化尤为明显。已尝试将染色模式与通过薄层色谱法和硫酸灰分分析揭示的染色剂成分相关联。通过这种方式,已能够确定获得满意染色效果的一些基本要求。

相似文献

1
An evaluation of some commerical Romanowsky stains.一些商用罗曼诺夫斯基染色剂的评估
J Clin Pathol. 1975 Aug;28(8):680-5. doi: 10.1136/jcp.28.8.680.
2
Romanowsky staining in cytopathology: history, advantages and limitations.细胞病理学中的罗曼诺夫斯基染色:历史、优点与局限性
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A rapid thin-layer chromatographic system for Romanowsky blood stains.一种用于罗曼诺夫斯基血迹的快速薄层色谱系统。
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Standardization of the Romanowsky staining procedure: an overview.罗曼诺夫斯基染色程序的标准化:概述
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Refractile mycobacteria in Romanowsky-stained bone marrow smears. A comparison of acid-fast-stained tissue sections and Romanowsky-stained smears.罗曼诺夫斯基染色骨髓涂片可见折光性分枝杆菌。抗酸染色组织切片与罗曼诺夫斯基染色涂片的比较。
Am J Clin Pathol. 1992 Mar;97(3):318-21. doi: 10.1093/ajcp/97.3.318.
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ICSH reference method for staining of blood and bone marrow films by azure B and eosin Y (Romanowsky stain). International Committee for Standardization in Haematology.国际血液学标准化委员会关于用天青B和伊红Y对血液和骨髓涂片进行染色的参考方法(罗曼诺夫斯基染色法)。
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Romanowsky staining, the Romanowsky effect and thoughts on the question of scientific priority.罗曼诺夫斯基染色法、罗曼诺夫斯基效应以及关于科学优先权问题的思考
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Microsc Acta. 1977 Mar;79(2):153-6.
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How Romanowsky stains work and why they remain valuable - including a proposed universal Romanowsky staining mechanism and a rational troubleshooting scheme.罗曼诺夫斯基染色法的工作原理及其为何依然具有重要价值——包括一种提出的通用罗曼诺夫斯基染色机制和一种合理的故障排除方案。
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Wright-Giemsa and nonspecific esterase staining of cells.细胞的瑞氏-吉姆萨染色和非特异性酯酶染色。
Curr Protoc Cytom. 2001 May;Appendix 3:Appendix 3D. doi: 10.1002/0471142956.cya03ds11.

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[Spectroscopic and thermodynamic investigations on the binding of azure B to chondroitin sulfate and the structure of the metachromatic dye complex].[天青B与硫酸软骨素结合及异染性染料复合物结构的光谱和热力学研究]
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Standardization of biological dyes and stains: pitfalls and possibilities.生物染料和染色剂的标准化:陷阱与可能性
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A standardized Romanowsky stain prepared from purified dyes.一种由纯化染料制备的标准化罗曼诺夫斯基染色剂。
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Romanowsky-type stains in haematology.血液学中的罗曼诺夫斯基氏染色法。
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本文引用的文献

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[Physico-chemical characteristics of commercial thiazine dyes. 2. Qualitative and quantitative investigations on the impurity of the dye stuffs and the "unsolubility" for the achievement of higher purity].
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An improved method for Giemsa staining of formalin-fixed tissue sections.
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Batch variations in commercial dyes employed for Romanowsky-type staining: a thin-layer chromatographic study.用于罗曼诺夫斯基型染色的商用染料中的批次差异:一项薄层色谱研究。
Stain Technol. 1974 Nov;49(6):351-8. doi: 10.3109/10520297409117011.
4
The influence of salt on the staining of tissue sections with basic dyes: an investigation into the general applicability of the critical electrolyte concentration theory.盐对碱性染料染色组织切片的影响:对临界电解质浓度理论普遍适用性的研究
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A rapid thin-layer chromatographic system for Romanowsky blood stains.一种用于罗曼诺夫斯基血迹的快速薄层色谱系统。
Stain Technol. 1974 Jul;49(4):235-40. doi: 10.3109/10520297409116984.
6
Some effects of salts on staining: use of the Donnan equilibrium to describe staining of tissue sections with acid and basic dyes.盐类对染色的某些影响:利用唐南平衡描述组织切片用酸性和碱性染料的染色情况。
Histochemistry. 1974 Mar 27;39(1):71-82. doi: 10.1007/BF02428553.
7
The Giemsa stain for tissue sections: an improved method.
Am J Clin Pathol. 1973 Aug;60(2):148-56. doi: 10.1093/ajcp/60.2.148.