鸡胚核糖体晶体：体外结合与功能活性分析

Proc Natl Acad Sci U S A. 1971 Feb;68(2):440-4. doi: 10.1073/pnas.68.2.440.

The intracellular ribosome crystals from chick embryos incubated at 5 degrees C have been isolated; stable products of their dissociation were shown to include single ribosomes, tetramers of ribosomes, and tetramers of large subunits. Conditions were found for the formation in vitro not only of the p4 crystals from ribosomal tetramers but also of the tetramers themselves from free ribosomes. The polypeptide synthesizing activity of these ribosomes was found to be unimpaired by their constraint in the tetrameric configuration.

已分离出在5摄氏度下孵育的鸡胚细胞内核糖体晶体；其解离的稳定产物包括单个核糖体、核糖体四聚体和大亚基四聚体。已发现体外不仅能由核糖体四聚体形成p4晶体，还能由游离核糖体形成四聚体本身的条件。发现这些核糖体的多肽合成活性不受其在四聚体构型中的限制影响。