Pascal C, Gaillard C, Moreau M O
J Assoc Off Anal Chem. 1979 Sep;62(5):976-81.
Nosiheptic is determined in fermentation broths of Streptomyces actuosus either by a microbiological method using Staphylococcus aureus or, more easily, by an automated colorimetric method. The results obtained with both methods correspond well for concentrations greater than 100 microgram/mL with a standard deviation of 1-3%. For determination of nosiheptide as a feed additive, the microbiological assay is made more specific by pretreatment with petroleum ether and 1N HCl. Standard deviation is less than 4%, and the assay is sensitive to 1 ppm. Nosiheptide is identified in feed containing other frequently used antibiotics by thin layer chromatography with bioautography; sensitivity is 1 ppm. The absence of traces of nosiheptide in tissues of treated swine and broiler is confirmed by microbiological diffusion, sensitive to 0.025 ppm.
诺西肽可通过使用金黄色葡萄球菌的微生物学方法或更简便的自动比色法在活跃链霉菌发酵液中测定。对于浓度大于100微克/毫升的情况，两种方法得到的结果吻合良好，标准偏差为1 - 3%。为了测定作为饲料添加剂的诺西肽，通过用石油醚和1N盐酸预处理可使微生物学测定更具特异性。标准偏差小于4%，该测定对1 ppm敏感。通过带有生物自显影的薄层色谱法在含有其他常用抗生素的饲料中鉴定诺西肽；灵敏度为1 ppm。通过对0.025 ppm敏感的微生物扩散法证实，经处理的猪和肉鸡组织中不存在诺西肽痕迹。
