采用气相色谱法分析人体脂质中的脂肪酸。
Analysis of fatty acids from human lipids by gas chromatography.
作者信息
Haan G J, van der Heide S, Wolthers B G
出版信息
J Chromatogr. 1979 Mar 1;162(3):261-71. doi: 10.1016/s0378-4347(00)81513-3.
A rapid, quantitative method is described for the analysis of fatty acids from human lipids, namely serum lipids and lipids from adipose tissue biopsies. The method includes extraction of serum lipids with chloroform--methanol, hydrolysis with tetramethylammonium hydroxide, methylation with methyl iodide and N,N-dimethylformamide and gas chromatographic analysis on a Supelcoport SP-2320 column. Fat biopsies are analysed without extraction. Optimal hydrolysis conditions have been investigated.
描述了一种快速、定量分析人脂质中脂肪酸的方法，即血清脂质和脂肪组织活检样本中的脂质。该方法包括用氯仿 - 甲醇提取血清脂质，用氢氧化四甲铵水解，用碘甲烷和N，N - 二甲基甲酰胺甲基化，以及在Supelcoport SP - 2320柱上进行气相色谱分析。脂肪活检样本无需提取即可进行分析。已研究了最佳水解条件。
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