采用高效液相色谱-电化学检测法测定血液中的吗啡及其主要代谢物吗啡-3-葡萄糖醛酸苷。

Determination of morphine and its major metabolite, morphine-3-glucuronide, in blood by high-performance liquid chromatography with electrochemical detection.

作者信息

White M W

出版信息

J Chromatogr. 1979 Oct 19;178(1):229-40. doi: 10.1016/s0021-9673(00)89711-3.

DOI:10.1016/s0021-9673(00)89711-3

Abstract

The construction of an electrochemical detector flow cell for high-performance liquid chromatography from stainless steel, which also serves as the auxiliary electrode, is described. Glassy carbon is used for the working electrode in a novel configuration based on a hybrid of the wall-jet and thin-layer arrangements. Application of the detector to the determination of morphine in small blood samples is described. The metabolite, morphine-3-glucuronide, is also determined by hydrolysis with beta-glucoronidase prior to extraction. The detector is capable of detecting less than 1 ng of morphine injected.

摘要

描述了一种用于高效液相色谱的电化学检测器流通池的构建，该流通池由不锈钢制成，同时用作辅助电极。玻碳用于工作电极，采用了基于壁流喷射和薄层配置混合的新颖结构。描述了该检测器在测定小血样中吗啡的应用。代谢物吗啡 - 3 - 葡萄糖醛酸苷在提取前也通过β - 葡萄糖醛酸酶水解来测定。该检测器能够检测到注入的不到1 ng的吗啡。

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