Oppenheim J J, Whang J, Frei E
J Exp Med. 1965 Oct 1;122(4):651-64. doi: 10.1084/jem.122.4.651.
The lymphocyte proliferation in repeatedly studied mixed leukocyte cultures of peripheral white blood cells from a skin graft donor and 2 recipients was significantly increased at the time of graft rejection. This was determined from the increased proportions of mononuclear cells labeling with tritiated thymidine, increased mitotic indices, and the appearance of increased numbers of transformed lymphocytes after rejection of 1st and 2nd skin grafts. The temporarily enhanced response occurred sooner and was of shorter duration after the second than after the first graft, but was quantitatively similar each time. The cell proliferation in the mixed leukocyte cultures of the two recipients was similarly affected by the homograft rejections. The cultures containing three cell populations usually manifested a greater lymphocyte response than corresponding cultures of leukocytes from only two unrelated subjects. An increase in the ratio of female recipient to male graft donor metaphases in the cultures at the time of enhanced lymphocyte transformation indicated that proliferation of the graft recipient lymphocytes was responsible for the above findings. Unmixed, unstimulated control cultures grown in autologous, the other subjects plasma, or heterologous calf serum failed to support significant lymphocyte transformation. The role of humoral factors and relationship of the in vitro cellular responses to the in vivo homograft reaction are discussed.
在对皮肤移植供体及两名受体的外周血白细胞反复进行混合白细胞培养时发现，在移植排斥发生时，淋巴细胞增殖显著增加。这是通过氚标记胸腺嘧啶核苷标记的单核细胞比例增加、有丝分裂指数升高以及在首次和第二次皮肤移植排斥后转化淋巴细胞数量增加来确定的。第二次移植后，这种暂时增强的反应出现得更快且持续时间更短，但每次在数量上相似。两名受体的混合白细胞培养中的细胞增殖同样受到同种异体移植排斥的影响。含有三个细胞群体的培养物通常比仅来自两个不相关个体的白细胞相应培养物表现出更强的淋巴细胞反应。在淋巴细胞转化增强时，培养物中雌性受体与雄性移植供体中期细胞的比例增加，表明移植受体淋巴细胞的增殖是上述结果的原因。在自体血浆、其他受试者血浆或异种小牛血清中培养的未混合、未刺激的对照培养物未能支持显著的淋巴细胞转化。本文讨论了体液因子的作用以及体外细胞反应与体内同种异体移植反应的关系。
