Martin G R, Warocquier R, Boulanger P A
Intervirology. 1975;5(3-4):162-72. doi: 10.1159/000149893.
The two-dimensional immunoelectrophoresis technique was applied to adenovirus type 2 soluble antigens. Electrophoresis of adenovirus-infected HeLa cell extracts in adenovirus antibody-containing agarose gel permitted us to assay for hexon, assembled penton, free penton base and free fiber simultaneously in one single experiment. This technique was routinely used to characterize the adenovirus type 2 temperature-sensitive mutants.
二维免疫电泳技术应用于2型腺病毒可溶性抗原。在含腺病毒抗体的琼脂糖凝胶中对腺病毒感染的HeLa细胞提取物进行电泳，使我们能够在一次实验中同时检测六邻体、组装的五邻体、游离的五邻体基座和游离的纤维。该技术常规用于鉴定2型腺病毒温度敏感突变体。
