Cejková J, Lojda Z, Obenberger J, Havránková E
Histochemistry. 1975 Sep 7;45(1):71-5. doi: 10.1007/BF00508053.
In alkali burned rabbit cornea the stainability of glycosaminoglycans in cold microtome setions was investigated. Staining by Alcian blue in 3% acetic acid, Alcian blue in various MgCl2 concentration and toluidine blue (pH 4.5) was employed. From the 1st to the 4th experimental day the intensity of reactions was decreased. This is most probably due to an increased hydration of the corneal stroma. On the 7th day hydration was markedly suppressed and reached nearly the normal level. In this time interval a decreased stainability of glycosaminoglycans was seen accompanied by a complete loss of staining in the marginal zone. On the 14th day the stainability in the traumatized area began to restore and in the marginal zone appeared. On the 32nd day the staining intensity of both areas was normalised, however when lower concentrations of MgCl2 were used; in the presence of higher concentrations of MgCl2 the decreased staining intensity persisted and points to a lower sulfatation of glycosaminoglycans. This was particularly remarkable in the area bordering the injured zone. This decrease runs parallel to the increased activities of acid glycosidases (especially of acid beta-galactosidase) which were reported previously.
研究了碱烧伤兔角膜中糖胺聚糖在冷冻切片中的可染性。采用3%醋酸中的阿尔辛蓝、不同氯化镁浓度下的阿尔辛蓝以及甲苯胺蓝（pH 4.5）进行染色。从实验第1天到第4天，反应强度降低。这很可能是由于角膜基质水合作用增加所致。在第7天，水合作用明显受到抑制，几乎恢复到正常水平。在此时间间隔内，可见糖胺聚糖的可染性降低，同时边缘区完全失去染色。在第14天，创伤区域的可染性开始恢复，边缘区也出现可染性。在第32天，两个区域的染色强度恢复正常，然而当使用较低浓度的氯化镁时；在较高浓度的氯化镁存在下，染色强度降低持续存在，表明糖胺聚糖的硫酸化程度较低。这在与损伤区域相邻的区域尤为明显。这种降低与先前报道的酸性糖苷酶（尤其是酸性β-半乳糖苷酶）活性增加平行。
