通过冻融-溶菌酶裂解从大肠杆菌中提取的多核糖体。
Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis.
作者信息
Ron E Z, Kohler R E, Davis B D
出版信息
Science. 1966 Sep 2;153(3740):1119-20. doi: 10.1126/science.153.3740.1119.
PMID:5331371
Abstract
Polysomes can be extracted from Escherichia coli by freezing and thawing in the presence of lysozyme, followed by treatment with sodium deoxycholate. The method is simple and convenient; the yields consistently high.
摘要
多核糖体可以通过在溶菌酶存在下进行冻融，然后用脱氧胆酸钠处理从大肠杆菌中提取出来。该方法简单方便，产量一直很高。
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