Wessman G E, Miller D J
Appl Microbiol. 1966 Jul;14(4):636-42. doi: 10.1128/am.14.4.636-642.1966.
Pasteurella pestis, harvested after 24 to 30 hr of growth in a casein hydrolysate medium at 26 C, was resuspended and shaken in 3% lactose-0.1 m phosphate buffer for 4 hr at the same temperature. Certain characteristics of these starved cells were compared with those of control cells. No differences in the amounts of cellular carbohydrate or lipid were detected. The concentrations of the principal free amino acids were greater in the shaken cells, except that they contained no measureable arginine, and the normally large pools of intracellular tricarboxylic acid cycle intermediates were reduced. Greater viable-cell counts resulted with the cells that were shaken in lactose buffer than with the control cells when each was incubated at 5 C for several weeks. However, the reduced viabilities were apparent losses caused by the formation of aggregates of cells. The clumping of cells was caused by the polymerization of extracellular nucleic acids, principally deoxyribonucleic acid, that were excreted by the cells. Cell clumping could be partially prevented by prior shaking of the suspended cells, which removed some of the deleterious material, or by the action of crystalline deoxyribonuclease.
鼠疫耶尔森菌在26℃的酪蛋白水解物培养基中生长24至30小时后收获，重悬于3%乳糖 - 0.1m磷酸盐缓冲液中，并在相同温度下振荡4小时。将这些饥饿细胞的某些特性与对照细胞的特性进行比较。未检测到细胞碳水化合物或脂质含量的差异。除了不含可测量的精氨酸外，振荡细胞中主要游离氨基酸的浓度更高，并且细胞内三羧酸循环中间体的正常大量储备减少。当在5℃孵育数周时，在乳糖缓冲液中振荡的细胞比对照细胞产生更高的活细胞计数。然而，活力降低是由细胞聚集体形成导致的明显损失。细胞聚集是由细胞分泌的细胞外核酸（主要是脱氧核糖核酸）聚合引起的。通过预先振荡悬浮细胞（去除一些有害物质）或通过结晶脱氧核糖核酸酶的作用，可以部分防止细胞聚集。
