Tago Y, Aida K
Appl Environ Microbiol. 1977 Sep;34(3):308-14. doi: 10.1128/aem.34.3.308-314.1977.
A bacterium isolated from activated sludge formed a visible floc and also produced an exoenzyme that could bring about deflocculation. Scanning electron microscopic examination revealed that the cells were embedded in a film mesh in the floc, which disappeared after treatment with the deflocculating enzyme. Polysaccharides isolated from the floc were fractionated into three fractions by diethylaminoethyl-Sephadex A-25 column chromatography, whereas those from the free cells were fractionated into only two fractions. The missing fraction was a mucopolysaccharide composed of glucosamine, glucose, mannose, galactose, and rhamnose and was hydrolyzed to oligosaccharides by the deflocculating enzyme. The other two fractions were resistant to the enzyme. These results show that the mesh structure of the floc is dependent on a mucopolysaccharide hydrolyzed by the deflocculating enzyme.
从活性污泥中分离出的一种细菌形成了可见的絮凝物，并且还产生了一种能导致解絮凝的胞外酶。扫描电子显微镜检查显示，细胞嵌入在絮凝物中的膜状网中，在用解絮凝酶处理后该网消失。通过二乙氨基乙基 - 葡聚糖A - 25柱色谱法将从絮凝物中分离出的多糖分为三个部分，而从游离细胞中分离出的多糖仅分为两个部分。缺失的部分是一种由氨基葡萄糖、葡萄糖、甘露糖、半乳糖和鼠李糖组成的粘多糖，并且被解絮凝酶水解为寡糖。另外两个部分对该酶具有抗性。这些结果表明，絮凝物的网状结构取决于被解絮凝酶水解的粘多糖。
