Marchini M, Strocchi R, Castellani P P, Riva R
Basic Appl Histochem. 1979;23(2):137-48.
Replicas of freeze-fractured collagen fibrils of peripheral and central parts of the annulus fibrosus of bovine intervertebral discs show microfibrils which run either arranged in parallel or in a helix with an inclination-angle ranging from 4 degrees to 8 degrees. According to results of freeze-fracutred tissues, thin sections of specimens treated with 4M guanidinium chloride show collagen fibrils with a parallel or a slightly wavy microfibrillar packing. Thin sections of specimens treated with alcian blue diluted in MgCl2 critical electrolyte solutions reveal interfibrillar proteoglycan particles with a filamentous shape in the peripheral zones of the annulus fibrosus and a predominant leaf-like appearance in the inner ones. These observations are discussed with reference to previous data concerning the variation in composition from the peripheral to the inner parts of the annulus fibrosus.
牛椎间盘纤维环外周和中央部分的冷冻断裂胶原纤维的复制品显示，微纤维呈平行排列或呈螺旋状排列，倾斜角度在4度至8度之间。根据冷冻断裂组织的结果，用4M氯化胍处理的标本薄片显示胶原纤维具有平行或轻微波浪状的微纤维排列。在MgCl2临界电解质溶液中稀释阿尔辛蓝处理的标本薄片显示，在纤维环外周区域有丝状的纤维间蛋白聚糖颗粒，而在内侧区域则主要呈叶状外观。结合先前关于纤维环从外周到内侧成分变化的数据对这些观察结果进行了讨论。
