高渗透压对大肠杆菌的保护作用，使其免受大肠菌素的致死效应。
Protection of Escherichia coli from the lethal effect of colicins by high osmotic pressure.
作者信息
Beppu R, Arima K
出版信息
J Bacteriol. 1967 Jan;93(1):80-5. doi: 10.1128/jb.93.1.80-85.1967.
Abstract
The sensitivity of Escherichia coli to the lethal effect of colicin E(2) was reduced by elevation of osmotic pressure of the incubation medium. Optimal protection of the cells from the lethal effect of colicin E(2) was achieved with 0.6 to 0.8 m NaCl or with 0.8 m sucrose containing 0.01 m MgSO(4). Under such conditions, the degradation of deoxyribonucleic acid caused by colicin E(2) was also suppressed markedly. It was concluded that a high concentration of sucrose with Mg(++) might prevent the action of the adsorbed colicin E(2). A similar protection was observed against the lethal effect of colicin K.
摘要
通过提高培养介质的渗透压可降低大肠杆菌对大肠杆菌素E(2)致死作用的敏感性。用含0.01 m MgSO(4)的0.6至0.8 m NaCl或0.8 m蔗糖可实现对细胞免受大肠杆菌素E(2)致死作用的最佳保护。在这种条件下，由大肠杆菌素E(2)引起的脱氧核糖核酸降解也被显著抑制。得出的结论是，高浓度的蔗糖与Mg(++)可能会阻止吸附的大肠杆菌素E(2)的作用。对大肠杆菌素K的致死作用也观察到了类似的保护作用。
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