无胸腺细菌对5-溴尿嘧啶的利用
Utilization of 5-bromouracil by thymineless bacteria.
作者信息
Hewitt R, Suit J C, Billen D
出版信息
J Bacteriol. 1967 Jan;93(1):86-9. doi: 10.1128/jb.93.1.86-89.1967.
Abstract
Several thymineless Escherichia coli strains have been examined for their ability to replicate their deoxyribonucleic acid when bromouracil is substituted for thymine. The procedure we describe was used to identify a thymineless strain with characteristics relatively favorable to its use in bromouracil labeling experiments. In addition, mutants with an "absolute" thymine requirement could be easily distinguished from one with a "leaky" thymine requirement.
摘要
已对几种无胸腺嘧啶的大肠杆菌菌株进行了检测，以研究当用溴尿嘧啶替代胸腺嘧啶时它们复制脱氧核糖核酸的能力。我们所描述的程序用于鉴定一种无胸腺嘧啶菌株，其特性相对有利于在溴尿嘧啶标记实验中使用。此外，对胸腺嘧啶有“绝对”需求的突变体可以很容易地与对胸腺嘧啶有“渗漏”需求的突变体区分开来。
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