枯草芽孢杆菌转化过程中供体与受体脱氧核糖核酸的整合
The integration of donor and recipient deoxyribonucleic acid during transformation of Bacillus subtilis.
作者信息
Ayad S R, Barker G R
出版信息
Biochem J. 1969 Jun;113(1):167-74. doi: 10.1042/bj1130167.
Abstract
- DNA labelled with 5-bromo[(3)H]uracil was used to transform auxotrophic strains of Bacillus subtilis. 2. After various times of incubation, DNA was extracted from the transformed culture and subjected to equilibrium sedimentation in caesium chloride gradients. 3. In addition to heavy donor DNA and light recipient DNA, a component with an intermediate density was found and is believed to consist of a biological hybrid of donor and recipient. 4. The component of intermediate density was isolated and found to possess activity in transformation derived from both donor and recipient strains. 5. Denaturation of the component of intermediate density followed by centrifugation gave only one component, indicating that integration had occurred in both strands of the recipient DNA. 6. No integrated band was observed after uptake by competent cells of B. subtilis of heavy DNA prepared from Escherichia coli.
摘要
- 用5-溴[³H]尿嘧啶标记的DNA用于转化枯草芽孢杆菌的营养缺陷型菌株。2. 经过不同时间的培养后，从转化培养物中提取DNA，并在氯化铯梯度中进行平衡沉降。3. 除了重的供体DNA和轻的受体DNA外，还发现了一种具有中间密度的成分，据信它由供体和受体的生物杂种组成。4. 分离出中间密度的成分，发现其具有来自供体和受体菌株的转化活性。5. 中间密度成分变性后离心仅得到一个成分，表明受体DNA的两条链均发生了整合。6. 用枯草芽孢杆菌感受态细胞摄取从大肠杆菌制备的重DNA后，未观察到整合带。
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本文引用的文献
1
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Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8. doi: 10.1073/pnas.44.10.1072.
2
Molecular fate of DNA in genetic transformation of Pneumococcus.肺炎球菌基因转化中DNA的分子命运
J Mol Biol. 1962 Jul;5:119-31. doi: 10.1016/s0022-2836(62)80067-9.
3
BIOCHEMICAL AND GENETIC STUDIES OF INTEGRATION AND RECOMBINATION IN BACILLUS SUBTILIS TRANSFORMATION.枯草芽孢杆菌转化中整合与重组的生化及遗传学研究
Genetics. 1964 Oct;50(4):717-38. doi: 10.1093/genetics/50.4.717.
4
ON THE MECHANISM OF DEOXYRIBONUCLEATE INTEGRATION IN PNEUMOCOCCAL TRANSFORMATION.关于肺炎球菌转化中脱氧核糖核酸整合的机制
Proc Natl Acad Sci U S A. 1964 Aug;52(2):412-9. doi: 10.1073/pnas.52.2.412.
5
ON THE MECHANISM OF GENETIC RECOMBINATION IN TRANSFORMING BACILLUS SUBTILIS.关于枯草芽孢杆菌转化过程中的基因重组机制
J Mol Biol. 1964 Jul;9:236-45. doi: 10.1016/s0022-2836(64)80103-0.
6
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J Bacteriol. 1963 Oct;86(4):785-96. doi: 10.1128/jb.86.4.785-796.1963.
7
Fractionation of nucleic acids with the methylated albumin column.用甲基化白蛋白柱对核酸进行分级分离。
J Mol Biol. 1962 Mar;4:161-72. doi: 10.1016/s0022-2836(62)80048-5.
8
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J Bacteriol. 1967 Jan;93(1):86-9. doi: 10.1128/jb.93.1.86-89.1967.
9
On the nature of recombinants formed during transformation in Hemophilus influenzae.关于流感嗜血杆菌转化过程中形成的重组体的性质。
J Gen Physiol. 1966 Jul;49(6):197-209. doi: 10.1085/jgp.49.6.197.
10
Fate of transforming DNA in the Haemophilus influenzae transformation system.流感嗜血杆菌转化系统中转化DNA的命运
J Mol Biol. 1965 Sep;13(2):554-70. doi: 10.1016/s0022-2836(65)80117-6.
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