从海德氏果蝇幼虫唾液腺大规模制备细胞核。
Mass preparation of nuclei from the larval salivary glands of Drosophila hydei.
作者信息
Boyd J B, Berendes H D, Boyd H
出版信息
J Cell Biol. 1968 Aug;38(2):369-76. doi: 10.1083/jcb.38.2.369.
Abstract
A method has been developed for isolating gram quantities of salivary glands from late third instar larvae of Drosophila hydei. The isolated glands have a normal appearance and incorporate RNA and DNA precursors normally. Nuclei can be isolated from these glands in 90% yield with the use of detergents. These nuclei contain morphologically normal giant polytene chromosomes.
摘要
已开发出一种从海德氏果蝇（Drosophila hydei）三龄晚期幼虫中分离出克级唾液腺的方法。分离出的腺体外观正常，能正常掺入RNA和DNA前体。使用去污剂可从这些腺体中以90%的产量分离出细胞核。这些细胞核含有形态正常的巨大多线染色体。
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