Franklin E C, Pras M
J Exp Med. 1969 Oct 1;130(4):797-808. doi: 10.1084/jem.130.4.797.
Eight preparations of soluble amyloid and degraded amyloid (DAM) were compared immunologically. Unlike amyloid fibrils, six of eight preparations of DAM proved to be relatively strong immunogens. Antisera to DAM reacted weakly or not at all with normal human serum or extracts of normal tissues, but were specifically reactive with amyloid fibrils or DAM. Comparative studies of DAM'S from eight different subjects showed some degree of cross-reactivity among them, yet demonstrated that they were not identical. Similar conclusions were obtained by quantitative precipitin and complement fixation analyses. Comparison of the amyloid fibrils with the homologous DAM by complement fixation and absorption studies demonstrated the existence in DAM of antigenic determinants that were lacking or inaccessible in the native fibrils. A search for amyloid precursors and antibodies to amyloid in the sera of 12 patients proved unsuccessful.
对8种可溶性淀粉样蛋白和降解淀粉样蛋白（DAM）制剂进行了免疫学比较。与淀粉样纤维不同，8种DAM制剂中有6种被证明是相对较强的免疫原。抗DAM血清与正常人血清或正常组织提取物反应微弱或根本不反应，但与淀粉样纤维或DAM具有特异性反应。对来自8个不同受试者的DAM进行的比较研究表明，它们之间存在一定程度的交叉反应，但也表明它们并不相同。通过定量沉淀和补体固定分析也得出了类似的结论。通过补体固定和吸收研究对淀粉样纤维与同源DAM进行比较，证明DAM中存在天然纤维中缺乏或无法接近的抗原决定簇。在12名患者的血清中寻找淀粉样前体和抗淀粉样蛋白抗体未获成功。
