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关于甲醛反应对网织红细胞核糖体某些光学和物理性质影响的研究。

A study of the effects of reaction with formaldehyde on some optical and physical properties of reticulocyte ribosomes.

作者信息

Cox R A

出版信息

Biochem J. 1969 Oct;114(4):743-51. doi: 10.1042/bj1140743.

DOI:10.1042/bj1140743
PMID:5343777
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1184961/
Abstract
  1. The optical rotatory dispersion and ultraviolet-absorption spectrum of ribosomal RNA in situ appear to be unchanged when the ribosome is dissociated into its RNA and protein moieties. 2. Reaction with 0.05% formaldehyde at 20 degrees for 2hr. ;fixes' ribosomes so that they remain intact in 1% sodium dodecyl sulphate. 3. The RNA moiety of the ribosome undergoes a conformational change when ribosomes in 8% formaldehyde are heated at 70 degrees for 10min. and cooled to 20 degrees . After this treatment no double-helical character can be detected, but neither the sedimentation coefficient nor the morphology of the ribosome determined by electron microscopy is altered. 4. It is concluded that the RNA moiety of reticulocyte ribosomes is freely accessible to formaldehyde.
摘要
  1. 核糖体解离成RNA和蛋白质部分时，核糖体RNA原位的旋光色散和紫外吸收光谱似乎未发生变化。2. 在20℃下用0.05%甲醛反应2小时，“固定”核糖体，使其在1%十二烷基硫酸钠中保持完整。3. 当8%甲醛中的核糖体在70℃加热10分钟后冷却至20℃时，核糖体的RNA部分会发生构象变化。经过这种处理后，无法检测到双螺旋特征，但沉降系数和通过电子显微镜测定的核糖体形态均未改变。4. 得出的结论是，网织红细胞核糖体的RNA部分可自由与甲醛接触。
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c96d/1184961/8d9346ce22ab/biochemj00693-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c96d/1184961/b154f81a58c5/biochemj00693-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c96d/1184961/8d9346ce22ab/biochemj00693-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c96d/1184961/b154f81a58c5/biochemj00693-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c96d/1184961/8d9346ce22ab/biochemj00693-0089-a.jpg

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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INTERMOLECULAR CROSS LINKING OF A PROTEIN IN THE CRYSTALLINE STATE: CARBOXYPEPTIDASE-A.处于晶体状态的蛋白质的分子间交联：羧肽酶A
兔网织红细胞核糖体的一种可能结构。模型构建练习。
Biochem J. 1969 Oct;114(4):769-74. doi: 10.1042/bj1140769.
4
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5
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Biochem J. 1970 Mar;117(1):101-18. doi: 10.1042/bj1170101.
6
Radioactive labelling of ribosomal proteins with reductive alkylation and its use in studying ribosome-cytosol interactions.用还原烷基化法对核糖体蛋白进行放射性标记及其在研究核糖体-胞质溶胶相互作用中的应用。
Biochem J. 1976 Dec 15;160(3):427-32. doi: 10.1042/bj1600427.
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