• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

影响培养的肾皮质细胞乳酸脱氢酶同工酶模式的因素。

Factors affecting the lactate dehydrogenase isoenzyme pattern of cultured kidney-cortex cells.

作者信息

Güttler F, Clausen J

出版信息

Biochem J. 1969 Oct;114(4):839-45. doi: 10.1042/bj1140839.

DOI:10.1042/bj1140839
PMID:5343794
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1184973/
Abstract
  1. The lactate dehydrogenase isoenzyme pattern of cultured calf kidney-cortex cells was correlated to growth phase, changes in oxygen supply, mean generation time and changes in nutritional supply. 2. During culture of free cells and intact explants the lactate dehydrogenase isoenzyme pattern changed towards a dominance of isoenzymes containing the M subunit. 3. Of the shift in monomer proportion, 58% occurred during the lag phase and 42% during the initial part of the exponential growth phase. During the stationary phase the shift in monomer proportion reversed slightly. It was possible to relate the observed shift in monomer proportion to the glycolytic rate. 4. Factors that depressed glycolysis decreased the shift in monomer proportion. Oxygen was found to limit the decrease in the H subunit/M subunit ratio caused by anaerobic culture in vitro. 5. The results obtained support the view that the altered lactate dehydrogenase isoenzyme pattern of urine in renal ischaemia may be explained by anaerobic changes in the lactate dehydrogenase isoenzyme pattern of cortical tubule cells.
摘要
  1. 培养的小牛肾皮质细胞的乳酸脱氢酶同工酶模式与生长阶段、氧气供应变化、平均世代时间以及营养供应变化相关。2. 在游离细胞和完整外植体培养过程中，乳酸脱氢酶同工酶模式向以含M亚基的同工酶占主导转变。3. 单体比例的变化中，58%发生在迟缓期，42%发生在指数生长期初期。在稳定期，单体比例的变化略有逆转。可以将观察到的单体比例变化与糖酵解速率联系起来。4. 抑制糖酵解的因素会减少单体比例的变化。发现氧气可限制体外厌氧培养导致的H亚基/M亚基比值的降低。5. 所得结果支持这样一种观点，即肾缺血时尿液中乳酸脱氢酶同工酶模式的改变可能由皮质肾小管细胞乳酸脱氢酶同工酶模式的厌氧变化来解释。
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2dd/1184973/9827f73df9b1/biochemj00693-0189-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2dd/1184973/bb4c50c1f1d7/biochemj00693-0188-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2dd/1184973/c236f8aecfef/biochemj00693-0188-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2dd/1184973/1c83d827fc07/biochemj00693-0188-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2dd/1184973/9827f73df9b1/biochemj00693-0189-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2dd/1184973/bb4c50c1f1d7/biochemj00693-0188-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2dd/1184973/c236f8aecfef/biochemj00693-0188-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2dd/1184973/1c83d827fc07/biochemj00693-0188-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2dd/1184973/9827f73df9b1/biochemj00693-0189-a.jpg

相似文献

1
Factors affecting the lactate dehydrogenase isoenzyme pattern of cultured kidney-cortex cells.影响培养的肾皮质细胞乳酸脱氢酶同工酶模式的因素。
Biochem J. 1969 Oct;114(4):839-45. doi: 10.1042/bj1140839.
2
Ultrathin-layer microelectrophoretic determination of lactate dehydrogenase isoenzymes in corneal and conjunctival epithelium of the cow.牛角膜和结膜上皮中乳酸脱氢酶同工酶的超薄层微电泳测定
Histochemistry. 1994 Apr;101(4):271-5. doi: 10.1007/BF00315914.
3
Lactate dehydrogenase isoenzyme patterns in human foetal tissues during development.人类胎儿组织发育过程中的乳酸脱氢酶同工酶谱
Indian J Physiol Pharmacol. 1986 Oct-Dec;30(4):271-9.
4
Prenatal changes of lactate dehydrogenase isoenzyme patterns and activity in bovine tissues.牛组织中乳酸脱氢酶同工酶谱和活性的产前变化。
Physiol Bohemoslov. 1976;25(6):551-9.
5
Impact of culture conditions, culture media volumes, and glucose content on metabolic properties of renal epithelial cell cultures. Are renal cells in tissue culture hypoxic?培养条件、培养基体积和葡萄糖含量对肾上皮细胞培养物代谢特性的影响。组织培养中的肾细胞是否缺氧？
Cell Physiol Biochem. 1999;9(3):150-72. doi: 10.1159/000016312.
6
Renal lactate dehydrogenase (LDH) isoenzyme pattern in short-term experimental obstructive nephropathy.短期实验性梗阻性肾病中的肾乳酸脱氢酶（LDH）同工酶谱
Invest Urol. 1979 Jul;17(1):46-9.
7
The excretion of lacate dehydrogenase in human urine after he ingestion of aspiin.摄入阿司匹林后人体尿液中乳酸脱氢酶的排泄情况。
Biochem J. 1969 Sep;114(2):197-202. doi: 10.1042/bj1140197.
8
Development of glial cells in primary cultures: energy metabolism and lactate dehydrogenase isoenzymes.原代培养中神经胶质细胞的发育：能量代谢与乳酸脱氢酶同工酶
Neurochem Res. 1980 Aug;5(8):847-54. doi: 10.1007/BF00965784.
9
[Methodology of studying lactate dehydrogenase isoenzyme systems in mice. II. Enzyme electrophoresis in polyacrylamide gel].[小鼠乳酸脱氢酶同工酶系统的研究方法。II. 聚丙烯酰胺凝胶中的酶电泳]
Vopr Med Khim. 1972 Nov-Dec;18(6):657-62.
10
Biochemical and enzymological study of lactate dehydrogenase isoenzymes from commercial quality control sera and several animal tissue sources.来自商业质量控制血清和几种动物组织来源的乳酸脱氢酶同工酶的生化和酶学研究。
Eur J Clin Chem Clin Biochem. 1995 Nov;33(11):865-8. doi: 10.1515/cclm.1995.33.11.865.

引用本文的文献

1
Changes in lipid pattern of HeLa cells exposed to immunoglobulin G and complement.暴露于免疫球蛋白G和补体的HeLa细胞脂质模式的变化
Biochem J. 1969 Dec;115(5):959-68. doi: 10.1042/bj1150959.
2
Phospholipid synthesis in HeLa cells exposed to immunoglobulin G and complement.暴露于免疫球蛋白G和补体的HeLa细胞中的磷脂合成
Biochem J. 1972 Jul;128(4):953-60. doi: 10.1042/bj1280953.
3
Ozone inhalation in rats: effects on alkaline phosphatase and lactic dehydrogenase isoenzymes in lavage and plasma.大鼠吸入臭氧：对灌洗液和血浆中碱性磷酸酶及乳酸脱氢酶同工酶的影响。

本文引用的文献

1
Lactate dehydrogenase isoenzymes of human semen.人类精液中的乳酸脱氢酶同工酶。
Biochem J. 1965 Nov;97(2):513-7. doi: 10.1042/bj0970513.
2
Sequential alterations of lactic dehydrogenase isozymes during embryonic development and in tissue culture.胚胎发育及组织培养过程中乳酸脱氢酶同工酶的序列变化
Proc Soc Exp Biol Med. 1962 Jul;110:582-5. doi: 10.3181/00379727-110-27586.
3
Succinic dehydrogenase and cytochrome oxidase activities in cell cultures.细胞培养物中的琥珀酸脱氢酶和细胞色素氧化酶活性
Bull Environ Contam Toxicol. 1988 Oct;41(4):502-7. doi: 10.1007/BF02020993.
J Biophys Biochem Cytol. 1960 Apr;7(2):265-72. doi: 10.1083/jcb.7.2.265.
4
The change in concentration of certain constituents of the medium during growth of the strain HeLa cells.海拉细胞系生长过程中培养基某些成分浓度的变化。
Am J Hyg. 1955 May;61(3):326-33. doi: 10.1093/oxfordjournals.aje.a119757.
5
PATHWAYS OF CARBOHYDRATE METABOLISM AND THEIR PHYSIOLOGICAL SIGNIFICANCE.碳水化合物代谢途径及其生理意义。
J Chem Educ. 1965 May;42:244-53. doi: 10.1021/ed042p244.
6
GROWTH AND GLYCOLYSIS IN THE HUMAN DIPLOID CELL STRAIN WI-38.人二倍体细胞株WI-38中的生长与糖酵解
Proc Soc Exp Biol Med. 1965 Apr;118:1109-13. doi: 10.3181/00379727-118-30057.
7
DEVELOPMENTAL CHANGES IN EMBRYONIC ENZYME PATTERNS: THE EFFECT OF OXIDATIVE SUBSTRATES ON LACTIC DEHYDROGENASE IN BEATING CHICK EMBRYONIC HEART CELL CULTURES.胚胎酶模式的发育变化：氧化底物对鸡胚胎心脏跳动细胞培养物中乳酸脱氢酶的影响。
Dev Biol. 1964 Jun;9:327-46. doi: 10.1016/0012-1606(64)90029-6.
8
DIVERSITY IN LACTIC DEHYDROGENASE ELECTROPHORETIC PATTERNS WITH HUMAN CELL CULTURES.人类细胞培养物中乳酸脱氢酶电泳图谱的多样性
Exp Cell Res. 1964 Feb;33:562-70. doi: 10.1016/0014-4827(64)90021-7.
9
A comparative study of respiratory metabolism in cultured mammalian cell strains.培养的哺乳动物细胞株呼吸代谢的比较研究。
Exp Cell Res. 1963 Apr;30:369-78. doi: 10.1016/0014-4827(63)90308-2.
10
Oxygen tension of the urine and renal structures. Preliminary report of clinical findings.尿液及肾脏结构的氧张力。临床研究初步报告。
N Engl J Med. 1963 Jul 18;269:115-21. doi: 10.1056/NEJM196307182690301.