从丽蝇（Calliphora vomitoria）大脑中分离出具有胰岛素样免疫生物学活性的物质。
Isolation of material displaying insulin-like immunological biological activity from the brain of the blowfly Calliphora vomitoria.
作者信息
Duve H, Thorpe A, Lazarus N R
出版信息
Biochem J. 1979 Nov 15;184(2):221-7. doi: 10.1042/bj1840221.
Abstract
An insulin-like material from the brain of the blowfly Calliphora vomitoria was partially purified by acid alcohol extraction, gel filtration and ion-exchange cellulose chromatography. In addition, the RF value on polyacrylamide-gel electrophoresis was determined. The material was characterized by its ability to cross-react with bovine insulin antibody and by displaying diminished immunoreactivity on dilution. It displaced specifically bound 125I-labelled insulin from rat liver plasma membrane insulin receptors and displayed insulin-like biological activity on the isolated rat fat-cell. Within 30 min of injection into Calliphora, made hypertrehalocaemic and hyperglucaemic as a result of median neurosecretory cell removal, it caused the concentrations of both sugars to return to normal. The hypothesis is put forward that the median neurosecretory cells are the source of the material.
摘要
通过酸醇提取、凝胶过滤和离子交换纤维素色谱法，对丽蝇（Calliphora vomitoria）脑中的一种胰岛素样物质进行了部分纯化。此外，还测定了其在聚丙烯酰胺凝胶电泳上的RF值。该物质的特征在于它能够与牛胰岛素抗体发生交叉反应，并且稀释后免疫反应性降低。它能特异性地从大鼠肝细胞膜胰岛素受体上取代结合的125I标记胰岛素，并在分离的大鼠脂肪细胞上表现出胰岛素样生物活性。在去除中脑神经分泌细胞后，丽蝇出现高海藻糖血症和高血糖症，注射该物质后30分钟内，两种糖的浓度都恢复到了正常水平。由此提出假说，中脑神经分泌细胞是该物质的来源。
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