丽蝇（Calliphora vomitoria）脑中胰多肽样物质的分离与部分特性分析
Isolation and partial characterization of pancreatic polypeptide-like material in the brain of the blowfly Calliphora vomitoria.
作者信息
Duve H, Thorpe A, Neville R, Lazarus N R
出版信息
Biochem J. 1981 Sep 1;197(3):767-70. doi: 10.1042/bj1970767.
Abstract
Using 10(6) flies (5 kg of heads) a pancreatic polypeptide-like material has been partially purified from the blowfly Calliphora vomitoria. The isolated material was eluted on Sephadex G-50 similarly to bovine pancreatic polypeptide and had an RF on polyacrylamide-gel electrophoresis that was identical with that of the bovine hormone. The material diluted linearly and showed parallelism with bovine standards in a bovine pancreatic polypeptide immunoassay. In specificity controls the immunoreactivity was not abolished by trasylol and no cross-reactivity was discerned in assay for glucagon, proangiotensin and cyclic AMP. These data suggest that the pancreatic polypeptide material in the brain of the blowfly has close structural similarity to the mammalian hormone.
摘要
利用10⁶只苍蝇（5千克蝇头），从反吐丽蝇中部分纯化出一种类胰多肽物质。分离出的物质在Sephadex G - 50上的洗脱情况与牛胰多肽相似，并且在聚丙烯酰胺凝胶电泳上的Rf值与牛激素的相同。该物质呈线性稀释，在牛胰多肽免疫测定中与牛标准品表现出平行关系。在特异性对照中，抑肽酶并未消除其免疫反应性，在胰高血糖素、血管紧张素原和环磷酸腺苷的测定中也未发现交叉反应。这些数据表明，反吐丽蝇脑中的胰多肽物质与哺乳动物激素在结构上有密切的相似性。
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