Kocourek J, Ballou C E
J Bacteriol. 1969 Dec;100(3):1175-81. doi: 10.1128/jb.100.3.1175-1181.1969.
Controlled acetolysis of yeast mannans yields mixtures of oligosaccharides with (1-->2) and (1-->3) linkages between the mannose units, whereas the less stable (1-->6) linkages of the polysaccharide backbone are cleaved. The "fingerprints," obtained by gel filtration of the oligosaccharide mixtures, can be used to distinguish between the different yeast mannans. The general method may be useful for determining the taxonomy of yeasts and for making correlations between immunochemical reactivity and mannan structure.
酵母甘露聚糖的可控乙酰解产生了在甘露糖单元之间具有(1→2)和(1→3)连接的寡糖混合物,而多糖主链中较不稳定的(1→6)连接则被切断。通过对寡糖混合物进行凝胶过滤获得的“指纹图谱”可用于区分不同的酵母甘露聚糖。该通用方法可能有助于确定酵母的分类学,并有助于建立免疫化学反应性与甘露聚糖结构之间的相关性。