Corbel M J
J Biol Stand. 1979;7(4):349-60. doi: 10.1016/s0092-1157(79)80009-8.
A phage for non-smooth cells of Brucella abortus was isolated from a mixture of three brucella-phages incubated with rough brucella cells in the presence of N-methyl-N'-nitro-N-nitrosoguanidine. It did not lyse smooth cells of Br. abortus strains nor those of other Brucella species, but during the course of its replication in rough organisms a small proportion of phage mutants were produced which were similar in properties to smooth-specific phages. The rough-specific phage, R, itself resembled Weybridge phage in its morphological and serological properties. Phage R was found to contain DNA and segregated into high and low density fractions, both with plaque-forming activity, on ultra-centrifugation in CsSO4 gradients. Unlike the smooth-specific phages it attached to heat-labile receptors present on non-smooth brucella cells.
从三种布鲁氏菌噬菌体与粗糙型布鲁氏菌细胞在N-甲基-N'-硝基-N-亚硝基胍存在下孵育的混合物中分离出一种针对流产布鲁氏菌非光滑细胞的噬菌体。它不能裂解流产布鲁氏菌菌株的光滑细胞，也不能裂解其他布鲁氏菌属物种的光滑细胞，但在其于粗糙型菌体内复制过程中，产生了一小部分噬菌体突变体，其特性与光滑特异性噬菌体相似。粗糙特异性噬菌体R本身在形态学和血清学特性上类似于韦布里奇噬菌体。发现噬菌体R含有DNA，在CsSO4梯度中超速离心时可分离成具有噬菌斑形成活性的高密度和低密度组分。与光滑特异性噬菌体不同，它附着于非光滑布鲁氏菌细胞上存在的热不稳定受体。
