Jones L M, Merz G S, Wilson J B
Appl Microbiol. 1968 Aug;16(8):1179-90. doi: 10.1128/am.16.8.1179-1190.1968.
The nature of the phage typing reactions on Brucella species was determined by rates of adsorption and infection, one-step growth experiments, and susceptibility to lysis from without. The highest rates of adsorption and infection were obtained on smooth B. abortus cultures, and large clear plaques were produced. One or a few phage particles per B. neotomae cell killed about one-half of the cells, but some went through an infective cycle and released mature phage that resulted in production of small clear plaques. With B. suis, more phage particles per cell were required to kill, replication did not occur, and plaques were not observed. Still greater numbers of phage particles were required to cause some inhibition of growth of B. melitensis lawns. Rough Brucella cultures and species, such as B. ovis and B. canis, were not affected by the highest concentrations of phage. B. abortus cultures of intermediate colonial morphology adsorbed phage, but only a few infected cells (after a delayed latent period) released mature phage. An infected culture or colony appeared normal until spontaneous phage mutants appeared which could penetrate the cell wall more effectively than the parent phage. The mutant phage multiplied more rapidly, and the colony changed to a sticky white form.
通过吸附和感染率、一步生长实验以及对体外裂解的敏感性，确定了噬菌体对布鲁氏菌属的分型反应性质。在光滑型流产布鲁氏菌培养物上获得了最高的吸附和感染率，并产生了大的清亮噬菌斑。每只新墨西哥布鲁氏菌细胞中有一个或几个噬菌体颗粒可杀死约一半的细胞，但有些细胞经历了感染周期并释放出成熟噬菌体，导致产生小的清亮噬菌斑。对于猪布鲁氏菌，每个细胞需要更多的噬菌体颗粒才能杀死，不会发生复制，也未观察到噬菌斑。需要更多数量的噬菌体颗粒才能对马尔他布鲁氏菌菌苔的生长产生一定抑制作用。粗糙型布鲁氏菌培养物和种类，如绵羊布鲁氏菌和犬布鲁氏菌，不受最高浓度噬菌体的影响。具有中间菌落形态的流产布鲁氏菌培养物可吸附噬菌体，但只有少数受感染细胞（经过延迟潜伏期后）释放出成熟噬菌体。受感染的培养物或菌落看起来正常，直到出现自发的噬菌体突变体，其穿透细胞壁的能力比亲本噬菌体更强。突变噬菌体繁殖更快，菌落变为粘性白色形态。
