Lie J T, Ungar B, Cowling D C
J Clin Pathol. 1969 Sep;22(5):554-7. doi: 10.1136/jcp.22.5.554.
Antimicrobial agents in the serum may affect the results of the Euglena method of serum vitamin B(12) assay. Sulphonamides suppress the growth of Euglena in concentrations attainable in the serum during treatment; streptomycin, chlortetracycline, erythromycin, kanamycin, and nitrofurantoin bleach Euglena but only when present in concentrations far exceeding the normal peak therapeutic blood levels. False low results of serum vitamin B(12) assay due to inhibitory and/or bleaching substances in the serum can be readily detected by microscopy of the assay cultures and Euglena cell counts.
血清中的抗菌药物可能会影响血清维生素B12测定的眼虫法结果。磺胺类药物在治疗期间血清中可达到的浓度下会抑制眼虫生长；链霉素、金霉素、红霉素、卡那霉素和呋喃妥因会使眼虫褪色，但只有当它们的浓度远远超过正常治疗血药峰值水平时才会如此。通过对测定培养物进行显微镜检查和眼虫细胞计数，可以很容易地检测出血清中由于抑制性和/或褪色物质导致的血清维生素B12测定结果假性偏低。
