抗菌剂对血清维生素B12检测中眼虫法的影响。

Effect of antimicrobial agents on the Euglena method of serum vitamin B12 assay.

作者信息

Lie J T, Ungar B, Cowling D C

出版信息

J Clin Pathol. 1969 Sep;22(5):554-7. doi: 10.1136/jcp.22.5.554.

DOI:10.1136/jcp.22.5.554

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC474246/

Abstract

Antimicrobial agents in the serum may affect the results of the Euglena method of serum vitamin B(12) assay. Sulphonamides suppress the growth of Euglena in concentrations attainable in the serum during treatment; streptomycin, chlortetracycline, erythromycin, kanamycin, and nitrofurantoin bleach Euglena but only when present in concentrations far exceeding the normal peak therapeutic blood levels. False low results of serum vitamin B(12) assay due to inhibitory and/or bleaching substances in the serum can be readily detected by microscopy of the assay cultures and Euglena cell counts.

摘要

血清中的抗菌药物可能会影响血清维生素B12测定的眼虫法结果。磺胺类药物在治疗期间血清中可达到的浓度下会抑制眼虫生长；链霉素、金霉素、红霉素、卡那霉素和呋喃妥因会使眼虫褪色，但只有当它们的浓度远远超过正常治疗血药峰值水平时才会如此。通过对测定培养物进行显微镜检查和眼虫细胞计数，可以很容易地检测出血清中由于抑制性和/或褪色物质导致的血清维生素B12测定结果假性偏低。

相似文献

1

Effect of antimicrobial agents on the Euglena method of serum vitamin B12 assay.抗菌剂对血清维生素B12检测中眼虫法的影响。

J Clin Pathol. 1969 Sep;22(5):554-7. doi: 10.1136/jcp.22.5.554.

2

Activities of various cobalamins for Euglena gracilis with reference to vitamin B 12 assay with Euglena.各种钴胺素对纤细裸藻的活性及与纤细裸藻维生素B12测定的关系。

J Clin Pathol. 1971 Feb;24(1):15-7. doi: 10.1136/jcp.24.1.15.

3

INVESTIGATIONS INTO THE EUGLENA METHOD FOR THE ASSAY OF THE VITAMIN B12 IN SERUM.血清中维生素B12测定的眼虫法研究

J Clin Pathol. 1964 Jan;17(1):14-26. doi: 10.1136/jcp.17.1.14.

4

An improved Euglena gracilis method for assay of serum vitamin B12 activity.

Indian J Med Sci. 1957 Mar;11(3):155-62.

5

Total serum vitamin B12 concentration in normal human adult serum assayed by Euglena gracilis.用纤细裸藻测定正常成人血清中总血清维生素B12浓度。

Clin Sci. 1959 Aug;18:389-98.

6

[Micromethod of vitamin B12 determination in the blood with protozoa Euglena gracilis Z].[利用纤细裸藻（Euglena gracilis Z）测定血液中维生素B12的微量方法]

Rocz Panstw Zakl Hig. 1969;20(5):543-9.

7

Studies with a more rapid method of vitamin B12 assay utilizing Euglena gracilis.使用纤细裸藻对维生素B12进行更快速测定方法的研究。

J Clin Pathol. 1959 Mar;12(2):153-6. doi: 10.1136/jcp.12.2.153.

8

The serum vitamin B12 level: its assay and significance.血清维生素B12水平：其测定方法及意义。

Clin Haematol. 1976 Oct;5(3):521-46.

9

Effect of gibberellic acid on the growth of Euglena gracilis as employed for the assay of vitamin B12.赤霉素对用于维生素B12测定的纤细裸藻生长的影响。

Bull Calcutta Sch Trop Med. 1962 Jul;10:111-3.

10

Plasma and erythrocyte vitamin B12 activity assayed by E. gracilis in health, polycythaemia and megaloblastic anaemia.通过纤细裸藻测定健康人、红细胞增多症患者和巨幼细胞贫血患者的血浆及红细胞维生素B₁₂活性。

Br J Haematol. 1968 Sep;15(3):325.

引用本文的文献

1

Problems in the diagnosis and investigation of megaloblastic anemia.巨幼细胞贫血的诊断与检查中的问题

Can Med Assoc J. 1980 May 10;122(9):999-1004.

2

[Psychoses in B12-avitaminoses without anemia].[无贫血的维生素B12缺乏症中的精神病]

Arch Psychiatr Nervenkr (1970). 1971;214(3):291-300. doi: 10.1007/BF00342631.

本文引用的文献

1

Chloroplasts of Euglena gracilis Affected by Furadantin.受痢特灵影响的衣藻叶绿体。

Science. 1962 Jul 20;137(3525):225-6. doi: 10.1126/science.137.3525.225.

2

INVESTIGATIONS INTO THE EUGLENA METHOD FOR THE ASSAY OF THE VITAMIN B12 IN SERUM.血清中维生素B12测定的眼虫法研究

J Clin Pathol. 1964 Jan;17(1):14-26. doi: 10.1136/jcp.17.1.14.

3

Bleaching Euglena gracilis with antihistamines and streptomycin-type antibiotics.用抗组胺药和链霉素类抗生素对纤细裸藻进行漂白。

Arch Mikrobiol. 1962;42:46-55. doi: 10.1007/BF00425189.

4

Furadantin: human blood level and urinary concentration.呋喃妥因：人体血液水平及尿液浓度

South Med J. 1955 Feb;48(2):149-51. doi: 10.1097/00007611-195502000-00008.

5

The serum vitamin B12 concentration in pernicious anemia.恶性贫血患者的血清维生素B₁₂浓度

J Lab Clin Med. 1954 Nov;44(5):715-22.

6

Euglena and vitamina B12.

Ann N Y Acad Sci. 1953 Oct 14;56(5):818-30. doi: 10.1111/j.1749-6632.1953.tb30260.x.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。