酵母细胞壁合成
Yeast cell-wall synthesis.
作者信息
Sentandreu R, Northcote D H
出版信息
Biochem J. 1969 Nov;115(2):231-40. doi: 10.1042/bj1150231.
Abstract
- A study of wall synthesis has been made by following the incorporation of radioactive glucose and threonine into the cytoplasm and wall of yeast. 2. Both glucose and threonine are incorporated into a mannan glycopeptide. The glucose is also synthesized into a structural glucan of the wall. 3. The mannan glycopeptide contains high-molecular-weight mannan and low-molecular-weight mannose and oligosaccharide units composed of mannose. Both types of carbohydrate are attached to the peptide. The extent of radioactive incorporation into these different carbohydrate constituents of the glycopeptide remained constant during a pulse-chase experiment. No evidence of a sequential synthesis of oligosaccharides and high-molecular-weight mannan was obtained. 4. Cycloheximide inhibits the incorporation of threonine into the wall but only partially inhibits the incorporation of glucose. Thus not all the polysaccharide deposited into the wall is dependent on a simultaneous peptide synthesis and incorporation. 5. Protoplasts grown in an iso-osmotic medium secreted a mannan polymer that was probably a glycopeptide.
摘要
- 通过追踪放射性葡萄糖和苏氨酸掺入酵母细胞质和细胞壁的过程，对细胞壁合成进行了一项研究。2. 葡萄糖和苏氨酸都掺入了甘露聚糖糖肽中。葡萄糖还被合成到细胞壁的结构葡聚糖中。3. 甘露聚糖糖肽含有高分子量甘露聚糖以及由甘露糖组成的低分子量甘露糖和寡糖单元。这两种碳水化合物都与肽相连。在脉冲追踪实验中，放射性掺入到糖肽这些不同碳水化合物成分中的程度保持恒定。未获得寡糖和高分子量甘露聚糖顺序合成的证据。4. 环己酰亚胺抑制苏氨酸掺入细胞壁，但仅部分抑制葡萄糖的掺入。因此，并非所有沉积到细胞壁中的多糖都依赖于同时进行的肽合成和掺入。5. 在等渗培养基中生长的原生质体分泌出一种可能是糖肽的甘露聚糖聚合物。
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本文引用的文献
1
The chemical composition and structure of the yeast cell wall.
Biochem J. 1952 May;51(2):232-6. doi: 10.1042/bj0510232.
2
Studies on yeast metabolism. II. Synthesis of cell carbohydrates during glucose fermentation and its inhibition by azide.
Biochem J. 1952 Jan;50(3):303-10. doi: 10.1042/bj0500303.
3
Physical and chemical properties of polysaccharides and glycoproteins of the yeast-cell wall.
Biochem J. 1960 Apr;75(1):12-7. doi: 10.1042/bj0750012.
4
Glucomannan-protein complexes from cell walls of yeasts.
J Biol Chem. 1959 Sep;234:2281-5.
5
A method for isolating intact mitochondria and nuclei from the same homogenate, and the influence of mitochondrial destruction on the properties of cell nuclei.
J Biophys Biochem Cytol. 1955 Mar;1(2):139-53. doi: 10.1083/jcb.1.2.139.
6
MATING IN PSEUDOMONAS AERUGINOSA.
Nature. 1965 Feb 20;205:822. doi: 10.1038/205822a0.
7
THE ENZYMIC SYNTHESIS OF YEAST MANNAN.
Biochem Biophys Res Commun. 1963 Aug 20;12:504-9. doi: 10.1016/0006-291x(63)90324-3.
8
The uptake of some sulphur-containing amino acids by a brewer's yeast.
J Gen Microbiol. 1963 May;31:247-59. doi: 10.1099/00221287-31-2-247.
9
The acid phosphatase of yeast. Localization and secretion by protoplasts.
Biochim Biophys Acta. 1963 Feb 12;67:324-6. doi: 10.1016/0006-3002(63)91832-8.
10
Localization of melibiase in a strain of veast.
C R Trav Lab Carlsberg. 1959;31:272-81.
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