茄病镰刀菌的纤维素酶。酶复合物的解析
The cellulase of Fusarium solani. Resolution of the enzyme complex.
作者信息
Wood T M
出版信息
Biochem J. 1969 Nov;115(3):457-64. doi: 10.1042/bj1150457.
Abstract
- Culture filtrates from Fusarium solani were fractionated by ion-exchange chromatography on DEAE-Sephadex, followed by gel chromatography on Sephadex G-100, into a C(1) component, a C(x) component (CM-cellulase) and a beta-glucosidase (cellobiase) component. 2. The individual components showed little capacity for the solubilization of cotton fibre (cellulase activity), but when recombined in their original proportions 81% of the original cellulase activity was recovered. 3. The C(1) components of F. solani and Trichoderma koningii were similar in their pH optima, heat stabilities over the pH range 5-8 and elution volumes on Sephadex G-100. 4. The C(1) component of F. solani synergized with the C(x) component of T. koningii and conversely. 5. The C(1) and the beta-glucosidase components of F. solani were devoid of the swelling-factor (S-factor) activity associated with the C(x) component.
摘要
- 茄病镰刀菌的培养滤液先通过DEAE - 葡聚糖凝胶进行离子交换层析，再通过葡聚糖凝胶G - 100进行凝胶层析，分离为C(1)组分、C(x)组分（CM - 纤维素酶）和β - 葡萄糖苷酶（纤维二糖酶）组分。2. 各组分对棉纤维的溶解能力（纤维素酶活性）很低，但按其原始比例重新组合时，可恢复81%的原始纤维素酶活性。3. 茄病镰刀菌和康氏木霉的C(1)组分在最适pH、5 - 8 pH范围内的热稳定性以及在葡聚糖凝胶G - 100上的洗脱体积方面相似。4. 茄病镰刀菌的C(1)组分与康氏木霉的C(x)组分协同作用，反之亦然。5. 茄病镰刀菌的C(1)组分和β - 葡萄糖苷酶组分不具有与C(x)组分相关的膨胀因子（S - 因子）活性。
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