Sharma R M, Packer R A
Appl Microbiol. 1969 Oct;18(4):589-95. doi: 10.1128/am.18.4.589-595.1969.
A study conducted on 300 fecal samples from a cow and a pig, each artificially contaminated with approximately four Salmonella organisms revealed that, of the three enrichment broths used in conjunction with the three selective media, the maximum number of isolations were obtained with Brilliant Green MacConkey broth (BGMB), followed by those obtained with tetrathionate (TTB), and the least with selenite broth. The combination of BGMB with Brilliant Green-neutral red-lactose agar (BGNRLA), and TTB with desoxycholate citrate agar (DCA) gave an equal number of isolations. Of the three selective media used in conjunction with the three enrichment broths, the maximum number of recoveries were obtained on BGNRLA, followed by those on DCA, and least number of isolations on bismuth sulfite agar (BSA). The combination of selenite F broth-BSA appeared to be somewhat inhibitory for the growth of Salmonella organisms. Of the two selective media combinations, the DCA-BGNRLA combination yielded the highest number of isolations. The use of all three selective media gave still better results. Selenite and tetrathionate broths were found unsuitable for isolating Salmonella choleraesuis from feces. BGMB, containing 100 mug of Brilliant Green per ml, proved to be a useful enrichment medium for the isolation of this organism from cow and pig fecal samples, each inoculated with 16 organisms. Observations recorded seemed to suggest that contaminant bacteria perhaps outgrow and mask S. choleraesuis. An incubation period of 24 to 30 hr was found optimum for the three enrichment broths. A longer period was detrimental in the case of TTB but not with selenite broth and BGMB.
一项对来自一头牛和一头猪的300份粪便样本进行的研究，每份样本都人工接种了约4个沙门氏菌菌株，结果显示，在与三种选择性培养基联合使用的三种增菌肉汤中，使用亮绿麦康凯肉汤（BGMB）获得的分离菌数量最多，其次是硫代硫酸盐肉汤（TTB），而亚硒酸盐肉汤获得的最少。BGMB与亮绿中性红乳糖琼脂（BGNRLA）组合，以及TTB与柠檬酸盐脱氧胆酸盐琼脂（DCA）组合获得的分离菌数量相同。在与三种增菌肉汤联合使用的三种选择性培养基中，在BGNRLA上获得的回收率最高，其次是DCA，而在亚硫酸铋琼脂（BSA）上的分离菌数量最少。亚硒酸盐F肉汤-BSA组合似乎对沙门氏菌的生长有一定抑制作用。在两种选择性培养基组合中，DCA-BGNRLA组合获得的分离菌数量最多。使用所有三种选择性培养基效果更好。发现亚硒酸盐和硫代硫酸盐肉汤不适用于从粪便中分离猪霍乱沙门氏菌。每毫升含100微克亮绿的BGMB被证明是一种有用的增菌培养基，可用于从接种了16个菌株的牛和猪粪便样本中分离该菌。记录的观察结果似乎表明，污染菌可能生长超过并掩盖了猪霍乱沙门氏菌。发现24至30小时的培养时间对三种增菌肉汤来说是最佳的。培养时间延长对TTB不利，但对亚硒酸盐肉汤和BGMB则不然。
