Huhtanen C N, Naghski J
Appl Microbiol. 1972 Mar;23(3):578-85. doi: 10.1128/am.23.3.578-585.1972.
Twenty-five meat-and-bone meal samples were enriched with either selenite-cystine or tetrathionate and incubated for 1 and 2 days. Seven were previously found to be positive; of the other 18, 16 were positive for salmonella. The number of somatic serogroups per sample ranged from 1 to 11 with a mean of 3.8. Significantly more (P < 0.01) group C(1) salmonellae were isolated using tetrathionate than selenite, whereas significantly more of groups G, 35, and Difco poly-valent D were isolated from selenite than tetrathionate. Seventy-six percent of the presumptive colonies from Brilliant Green agar showed a positive lysine decarboxylase reaction, and there were no differences between media or times of incubation. Ninety-four per cent of the lysine decarboxylase-positive cultures showed a positive somatic antiserum response; again there were no differences between times or enrichments although there were significantly more total positive serogroups at 2 days than at 1 day from tetrathionate but not from selenite. There were indications that certain serogroups preferred either one or the other enrichment. There were no differences in total positive samples with the two enrichments although neither alone was sufficient to identify all positives. Several lactose-positive salmonellae were recovered.
将25份肉骨粉样本分别用亚硒酸盐 - 胱氨酸或连四硫酸盐进行增菌，并培养1天和2天。之前发现7份样本呈阳性；在其他18份样本中，16份沙门氏菌检测呈阳性。每个样本中体细胞血清群的数量范围为1至11，平均为3.8。使用连四硫酸盐分离出的C(1)群沙门氏菌显著多于（P < 0.01）使用亚硒酸盐分离出的；而从亚硒酸盐中分离出的G群、35群和Difco多价D群沙门氏菌显著多于从连四硫酸盐中分离出的。亮绿琼脂平板上76%的推测菌落显示赖氨酸脱羧酶反应呈阳性，不同培养基或培养时间之间没有差异。94%的赖氨酸脱羧酶阳性培养物显示体细胞抗血清反应呈阳性；同样，不同时间或增菌方法之间没有差异，尽管从连四硫酸盐增菌2天获得的总阳性血清群显著多于1天的，但从亚硒酸盐增菌的情况并非如此。有迹象表明某些血清群更喜欢其中一种增菌方法。两种增菌方法得到的总阳性样本数没有差异，尽管单独使用任何一种方法都不足以鉴定出所有阳性样本。还分离出了几株乳糖阳性沙门氏菌。
