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通过分析表皮碳氢化合物鉴别冈比亚按蚊吉尔斯复合体(双翅目:蚊科)的种类

Differentiation between species of the Anopheles gambiae Giles complex (Diptera: Culicidae) by analysis of cuticular hydrocarbons.

作者信息

Carlson D A, Service M W

出版信息

Ann Trop Med Parasitol. 1979 Dec;73(6):589-92. doi: 10.1080/00034983.1979.11687301.

DOI:10.1080/00034983.1979.11687301
PMID:539859
Abstract

Lipids were extracted from 500 female Anopheles gambiae and An. arabiensis in n-hexane and the extracts examined by gas chromatographic analysis using a glass column packed with 3% OV-1 on a 100-200 mesh Gas Chrom Q which was maintained at 225 degrees C. Cuticular hydrocarbons consisted mostly of unbranched paraffins. There were differences between the distribution of compounds with 25-29 carbons. After adjusting the C27 peak to 100%, hydrocarbons C26 and C29 represent heights of 80 and 69% in An. gambiae compared with values of 49 and 95 % in An. arabiensis. Other small but consistent differences were also found. Investigations are being performed on extracts from smaller samples and on other species within the An. gambiae complex.

摘要

从500只雌性冈比亚按蚊和阿拉伯按蚊中用正己烷提取脂质,并使用填充有3% OV - 1的100 - 200目Gas Chrom Q玻璃柱在225℃下进行气相色谱分析来检测提取物。表皮碳氢化合物主要由直链石蜡组成。含有25 - 29个碳的化合物分布存在差异。将C27峰调整为100%后,冈比亚按蚊中C26和C29碳氢化合物的峰高分别为80%和69%,而阿拉伯按蚊中分别为49%和95%。还发现了其他一些细微但一致的差异。目前正在对较小样本的提取物以及冈比亚按蚊复合体中的其他物种进行研究。

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