成纤维细胞向体外伤口的迁移。
The migration of fibroblasts into an in vitro wound.
作者信息
Stewart R J, Duley J A, Allardyce R A
出版信息
Br J Exp Pathol. 1979 Dec;60(6):582-8.
Abstract
A method for the study in vitro of fibroblast migration in wounds is described. This consists of producing standard fibroblast monolayers (derived from subcultures of explants of flank skin from young male rats) in microtitre plates, wounding the monolayer in a linear fashion with razor blade, removing the monolayer from one side of the linear wound and retaining the other side for migration studies. Preliminary results using the method are described which illustrate its reproducibility and ease of performance.
摘要
本文描述了一种体外研究伤口中成纤维细胞迁移的方法。该方法包括在微量滴定板中制备标准的成纤维细胞单层（取自年轻雄性大鼠胁腹皮肤外植体的传代培养物），用剃须刀片以线性方式划伤单层，从线性伤口的一侧移除单层，保留另一侧用于迁移研究。文中描述了使用该方法的初步结果，这些结果说明了该方法的可重复性和操作简便性。
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