大鼠腹水肝癌细胞中甲胎蛋白的合成
Synthesis of alpha-fetoprotein by rat ascites hepatoma cells.
作者信息
Kanai K, Endo Y, Oda T, Kaneko Y
出版信息
Ann N Y Acad Sci. 1975 Aug 22;259:29-36. doi: 10.1111/j.1749-6632.1975.tb25399.x.
AH-66 rat ascites hepatoma cells incorporated [14C]leucine into the AFP fraction. In a cell-free system, hepatoma ribosomes were found to be active in AFP synthesis whereas the supernatant fraction from hepatoma had no specific effect on AFP production. The amount of [14C]leucine incorporated in AFP by membrane-bound polysomes was 20 to 90 times higher than that by free polysomes, suggesting that AFP is mainly synthesized on membrane-bound polysomes. DBcAMP inhibited the growth of hepatoma cells. However, the incorporation of [14C]leucine into the AFP fraction as well as into total proteins was stimulated by DBcAMP.
AH-66大鼠腹水肝癌细胞将[14C]亮氨酸掺入甲胎蛋白组分中。在无细胞体系中，发现肝癌核糖体在甲胎蛋白合成中具有活性，而肝癌的上清液组分对甲胎蛋白的产生无特异性作用。膜结合多核糖体掺入甲胎蛋白中的[14C]亮氨酸量比游离多核糖体高20至90倍，这表明甲胎蛋白主要在膜结合多核糖体上合成。二丁酰环磷腺苷（DBcAMP）抑制肝癌细胞的生长。然而，DBcAMP刺激了[14C]亮氨酸掺入甲胎蛋白组分以及总蛋白中。
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