Moon M M, Redelman D, Wolvos T A, Bauer D C
J Immunol. 1977 Dec;119(6):2005-12.
Tolerance to hapten-specific antibody formation and delayed hypersensitivity was examined in adult rabbits. The azobenzenearsonate (ABA) or sulfonate-specific antibody response to hapten-hemocyanin immunogens was suppressed by deaggregated hapten-rabbit IgG conjugates given 21 and 14 days before challenge. High affinity antibody was preferentially suppressed. Delayed hypersensitivity to ABA-tyrosine was suppressed by deaggregated ABA-rabbit IgG conjugates injected 17 and 10 days before challenge. Conjugates with a high hapten density, ABA15-23-rabbit IgG were effective tolerogens. Conjugates with four to six ABA groups per carrier molecule were very poor tolerogens. Increasing the amount of low substituted conjugate injected did not improve tolerogenicity. It appears that a high epitope density is required for effective induction of tolerance to ABA-specific delayed hypersensitivity in the rabbit.
在成年兔中检测了对半抗原特异性抗体形成和迟发型超敏反应的耐受性。在攻击前21天和14天给予解聚的半抗原-兔IgG缀合物，可抑制对偶氮苯砷酸盐（ABA）或磺酸盐特异性抗体对半抗原-血蓝蛋白免疫原的反应。高亲和力抗体被优先抑制。在攻击前17天和10天注射解聚的ABA-兔IgG缀合物，可抑制对ABA-酪氨酸的迟发型超敏反应。具有高半抗原密度的缀合物ABA15-23-兔IgG是有效的耐受原。每个载体分子带有四至六个ABA基团的缀合物是非常差的耐受原。增加低取代缀合物的注射量并不能提高耐受性。看来，在兔中有效诱导对ABA特异性迟发型超敏反应的耐受性需要高表位密度。
