Wagner J, Palfreyman M, Zraika M
J Chromatogr. 1979 Sep 11;164(1):41-54. doi: 10.1016/s0378-4347(00)81570-4.
A method for the determination of catecholic amino acids and amines by reversed-phase ion-pair high-performance liquid chromatography with electrochemical detection has been developed. B using octanesulfonic acid for ion pairing and by optimising ionic strength, pH and methanol concentration of the mobile phase, separation was achieved of 3,4-dihydroxyphenylalanine (DOPA), 3,4-dihydroxyphenylacetic acid (DOPAC), norepinephrine (NE), epinephrine (EPI), and dopamine (DA). Alpha-Difluoromethyldopa (DFMD) and alpha-monofluoromethyldopa (MFMD), two potent enzyme-activated irreversible inhibitors of aromatic amino acid decarboxylase were also separated from the natural catechols. Concentrations of catechols and inhibitors were measured in brains, hearts and kidneys of mice treated with small repeated doses of MFMD. The method has also been applied to the determination of catechols in other organs such as prostates and seminal vesicles of rats and in smaller tissues like mesenteric arteries. A semi-automated procedure making use of an automatic sample processor and a digital integrator permitted the analysis of as many as sixty samples per day.
已开发出一种采用反相离子对高效液相色谱法并结合电化学检测来测定儿茶酚胺类氨基酸和胺类的方法。通过使用辛烷磺酸进行离子对作用，并优化流动相的离子强度、pH值和甲醇浓度，实现了对3,4 - 二羟基苯丙氨酸（DOPA）、3,4 - 二羟基苯乙酸（DOPAC）、去甲肾上腺素（NE）、肾上腺素（EPI）和多巴胺（DA）的分离。α - 二氟甲基多巴（DFMD）和α - 一氟甲基多巴（MFMD）这两种强效的芳香氨基酸脱羧酶的酶激活不可逆抑制剂也与天然儿茶酚类物质实现了分离。在用小剂量重复给药的MFMD处理的小鼠的脑、心脏和肾脏中测定了儿茶酚类物质和抑制剂的浓度。该方法还应用于测定其他器官中的儿茶酚类物质，如大鼠的前列腺和精囊，以及肠系膜动脉等较小组织中的儿茶酚类物质。利用自动进样器和数字积分仪的半自动程序允许每天分析多达60个样品。
