采用带电子捕获检测的气液色谱法测定溴己新的三氟乙酰衍生物。

Determination of bromhexine as its trifluoroacetyl derivative by gas-liquid chromatography with electron-capture detection.

作者信息

De Leenheer A P, Vandecasteele-Thienpont L M

出版信息

J Chromatogr. 1979 Jul 13;175(2):301-6. doi: 10.1016/s0021-9673(00)89436-4.

Abstract

A specific gas-liquid chromatographic method with electron-capture detection is described for the determination of bromhexine at the nanogram level. Structurally analogous internal standards were synthesized and their suitability was investigated, based on their chromatographic properties on different stationary liquid phases. The electron-capture activity of the compounds is increased by trifluoroacetylation. Reaction conditions for this derivatization were studied. Calibration graphs in the range 0-57 ng (in a total of 100 microliters of reaction mixture) showed good linearity.

摘要

描述了一种采用电子捕获检测的特定气-液色谱法，用于测定纳克级别的溴己新。合成了结构类似的内标物，并根据它们在不同固定液相上的色谱性质研究了其适用性。通过三氟乙酰化提高了化合物的电子捕获活性。研究了这种衍生化的反应条件。在0-57纳克范围内（总共100微升反应混合物中）的校准曲线显示出良好的线性。