Jonckheere J A, Thienpont L M, De Leenheer A P, De Backer P, Debackere M, Belpaire F M
Biomed Mass Spectrom. 1980 Nov;7(11-12):582-7. doi: 10.1002/bms.1200071127.
A method has been developed for quantification of bromhexine in plasma using gas chromatography mass spectrometry with selected ion monitoring. A deuterium labelled analogue was synthesized and used as the internal standard. To evaluate the gas chromatographic electron capture detection method described earlier, 23 plasma samples have been analysed by both techniques. Although a good correlation was shown, selected ion monitoring was superior to the electron capture detection method for levels below 3 ng ml-1. The mass spectrometric method has also been used to set up a pharmacokinetic study of bromhexine in horses. Urine extracts were subjected to gas chromatographic mass spectrometric analysis to identify the different metabolites of bromhexine. Three groups of metabolites were detected and identified.
已开发出一种使用气相色谱-质谱联用选择离子监测技术定量测定血浆中溴己新的方法。合成了一种氘标记类似物并用作内标。为评估先前描述的气相色谱电子捕获检测方法，用这两种技术分析了23份血浆样本。虽然显示出良好的相关性，但对于低于3 ng/ml的水平，选择离子监测优于电子捕获检测方法。质谱法也已用于建立马体内溴己新的药代动力学研究。对尿液提取物进行气相色谱-质谱分析以鉴定溴己新的不同代谢物。检测并鉴定出三组代谢物。
