Larson S M, Rasey J S, Allen D R, Grunbaum Z
J Nucl Med. 1979 Aug;20(8):843-6.
The EMT-6 sarcoma-like tumor of BALB/c mice can be grown as a solid subcutaneous transplantable tumor in vivo or as a monolayer culture in vitro. We have studied the uptake of gallium-67 by this tumor growing subcutaneously on the backs of 6-week-old BALB/c mice. After i.v. administration of Ga-67 citrate, tumor uptakes were as high as any others reported for mouse tumors. Also, for unknown reasons, there was appreciable reduction in tumor uptake with increasing amounts of Ga-67 citrate, even in the microcurie range. Furthermore, when mouse serum is prelabeled with Ga-67 and then injected, the EMT-6 uptake is greater than with Ga-67 administered as citrate (p less than 0.02). We believe that the finding of avid Ga-67 uptake in vivo helps to establish this unique in vivo/in vitro tumor system as a valid experimental model for studies regarding the mechanism of Ga-67 accumulation by neoplastic tissue.
BALB/c小鼠的EMT-6肉瘤样肿瘤可在体内作为实体皮下可移植肿瘤生长，也可在体外作为单层培养物生长。我们研究了这种在6周龄BALB/c小鼠背部皮下生长的肿瘤对镓-67的摄取情况。静脉注射柠檬酸镓-67后，该肿瘤的摄取量与报道的其他小鼠肿瘤一样高。此外，原因不明的是，即使在微居里范围内，随着柠檬酸镓-67剂量的增加，肿瘤摄取量也有明显降低。而且，当用镓-67预标记小鼠血清然后注射时，EMT-6肿瘤的摄取量比注射柠檬酸镓-67时更高（p<0.02）。我们认为，体内镓-67摄取量高这一发现有助于将这个独特的体内/体外肿瘤系统确立为研究肿瘤组织积累镓-67机制的有效实验模型。
