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用半自动荧光法对血清及血清脂蛋白组分中的胆固醇测定进行评估。

An evaluation of cholesterol determinations in serum and serum lipoprotein fractions by a semi-automated fluorimetric method.

作者信息

Robertson G, Cramp D G

出版信息

J Clin Pathol. 1970 Apr;23(3):243-5. doi: 10.1136/jcp.23.3.243.

DOI:10.1136/jcp.23.3.243
PMID:5420736
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC474531/
Abstract

A sensitive automated fluorimetric method for the estimation of cholesterol is described which is suitable for the routine determination of both serum and serum lipoprotein cholesterol. Analyses can be performed at 60/hour in the serum range of 5 mg to 400 mg/100 ml. The method shows a high degree of analytical precision and good yields from recovery experiments.

摘要

本文描述了一种灵敏的自动荧光法用于胆固醇的测定，该方法适用于血清和血清脂蛋白胆固醇的常规测定。在血清浓度为5mg至400mg/100ml的范围内，每小时可进行60次分析。该方法具有高度的分析精密度，回收实验的回收率良好。

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An evaluation of cholesterol determinations in serum and serum lipoprotein fractions by a semi-automated fluorimetric method.用半自动荧光法对血清及血清脂蛋白组分中的胆固醇测定进行评估。
J Clin Pathol. 1970 Apr;23(3):243-5. doi: 10.1136/jcp.23.3.243.
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引用本文的文献

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Studies of carbohydrate and lipid metabolism in women developing hypertension on oral contraceptives.关于服用口服避孕药的女性发生高血压时碳水化合物和脂质代谢的研究。
Br Med J. 1973 Aug 11;3(5875):317-20. doi: 10.1136/bmj.3.5875.317.
3
Lipid methodology.脂质测定方法。
J Clin Pathol Suppl (Assoc Clin Pathol). 1973;5:17-21. doi: 10.1136/jcp.s1-5.1.17.
4
Acipimox in the treatment of patients with hyperlipidaemia: a double blind trial.
Eur J Clin Pharmacol. 1986;31(2):201-4. doi: 10.1007/BF00606659.
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The effect of metoprolol on plasma lipids.美托洛尔对血脂的影响。
Postgrad Med J. 1979 Oct;55(648):709-11. doi: 10.1136/pgmj.55.648.709.
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Role of insulin resistance in adipose tissue and liver in the pathogenesis of endogenous hypertriglyceridaemia in man.胰岛素抵抗在人体内源性高甘油三酯血症发病机制中在脂肪组织和肝脏中的作用。
Diabetologia. 1976 Dec;12(6):563-71. doi: 10.1007/BF01220632.

本文引用的文献

1
A fluorometric method for total serum cholesterol.一种测定血清总胆固醇的荧光法。
J Lab Clin Med. 1957 Sep;50(3):485-8.
2
Estimation of total cholesterol in serum by a micro method.用微量法测定血清总胆固醇
Clin Chem. 1957 Aug;3(4 Pt 1):233-8.
3
The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum.人血清中超离心分离的脂蛋白的分布及化学组成
J Clin Invest. 1955 Sep;34(9):1345-53. doi: 10.1172/JCI103182.
4
The fluorometric assay of triglyceride by a semiautomated method.甘油三酯的半自动化荧光测定法。
Anal Biochem. 1968 Oct 24;25(1):246-51. doi: 10.1016/0003-2697(68)90097-3.