Horne C H, Herbert W J, White R G
Immunology. 1970 Apr;18(4):551-5.
Use of an intra-articular route of injection (a single, hind limb knee joint) and a protein antigen (human serum albumin) in water-in-oil emulsion with added mycobacterial peptidoglycolipid (or Wax D) failed to induce an adjuvant effect upon the resulting immunological response observed three weeks later. Serum antibody levels as measured by the Farr Test (ABC μg/ml) and cell-mediated immunity as measured by a quantitative corneal test were consistently lower than those which followed use of a footpad route for the same injection mixture.
采用关节内注射途径（单个后肢膝关节）并使用油包水乳液形式的蛋白质抗原（人血清白蛋白），同时添加分枝杆菌肽糖脂（或蜡质D），未能对三周后观察到的免疫反应产生佐剂效应。通过Farr试验（每毫升抗体活性单位）测定的血清抗体水平以及通过定量角膜试验测定的细胞介导免疫，始终低于对相同注射混合物采用足垫注射途径后的水平。
