Evaluation of the direct injection of antigen into a peripheral lymph node for the production of humoral and cell-mediated immunity in the guinea-pig.

The use of the intra-lymph node injection route for a range of doses of ovalbumin (antigen) and mycobacterial peptidoglycolipid (adjuvant) in water-in-oil emulsion failed to show any superiority of this method over the usual footpad injection, in terms of induction of cell-mediated hypersensitivity. The same result applied to anti-ovalbumin antibody levels in the serum at 3 weeks after injection, the findings with lower doses of ovalbumin were that lower levels of anti-ovalbumin resulted when the intra-lymph node injection route was used. For the induction of allergic encephalomyelitis the intra-lymph node injection route failed to prove superior to the footpad route at several dose-levels of mycobacterial peptidoglycolipid in water-in-oil emulsion with homologous brain tissue.