产气荚膜梭菌核酸和多糖的分离与分析。
Isolation and analysis of the nucleic acids and polysaccharides from Clostridium welchii.
作者信息
Darby G K, Jones A S, Kennedy J F, Walker R T
出版信息
J Bacteriol. 1970 Jul;103(1):159-65. doi: 10.1128/jb.103.1.159-165.1970.
Abstract
A method previously described for the use of bentonite in the isolation of the nucleic acids from two gram-positive organisms was applied to the isolation of the nucleic acids from two strains of Clostridium welchii. The nucleic acids were separated from polysaccharides by the fractional precipitation of their cetyltrimethyl-ammonium salts from sodium chloride solution, and the base composition of the nucleic acids was determined. One strain of C. welchii investigated (NCTC 10578) was shown to produce considerable quantities of an acidic and also a weakly acidic or neutral polysaccharide; the other strain (ATCC 10543) gave very small quantities of the latter but none of the former polysaccharide. The monosaccharide composition of these polysaccharides was determined and the acidic polysaccharide was shown to resemble dermatan sulfate.
摘要
一种先前描述的用于利用膨润土从两种革兰氏阳性菌中分离核酸的方法，被应用于从两株产气荚膜梭菌中分离核酸。通过从氯化钠溶液中分级沉淀核酸的十六烷基三甲基铵盐，将核酸与多糖分离，并测定了核酸的碱基组成。所研究的一株产气荚膜梭菌（NCTC 10578）被证明能产生大量的酸性多糖以及少量弱酸性或中性多糖；另一株（ATCC 10543）产生的后一种多糖量极少，而前一种多糖则未产生。测定了这些多糖的单糖组成，结果表明酸性多糖类似于硫酸皮肤素。
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