Hilgert I, Kristofová H, Rieger M
Folia Biol (Praha). 1975;21(6):409-13.
Cell-free spleen and liver extracts were used for attempted induction of allograft tolerance across a restricted H-2 barrier due to a chemically induced mutation in the B10.D2 mouse strain. When using mice of the latter strain as donors and the new congenic strain carrying the mutant H-2 hyaplotype as recipients, and with long-term administration of the cell-free antigens in adult life up to 100% yields (according to the dose of antigen) of permanently tolerant animals (particularly males) was obtained with spleen extract, but not with liver extract. This contrasts with a higher tolerance-inducing efficiency of liver extracts in various non-H-2 incompatibilities. In the reversed donor-recipient combination, neither spleen nor liver extracts gave positive results. The immune status of the permanently tolerant animals was analyzed and it was shown that with their bone marrow normal responsiveness can be conferred upon lethally irradiated syngeneic hosts whereas the specific allograft tolerance can be adoptively transferred to sublethally irradiated recipients by means of spleen or lymph node cells. It is concluded that in the present system the state of allograft tolerance is maintained by an immunologically active process of peripheral lymphocytes.
由于B10.D2小鼠品系中存在化学诱导突变，无细胞脾和肝提取物被用于尝试跨越有限的H-2屏障诱导同种异体移植耐受。当以后者品系的小鼠作为供体，携带突变H-2单倍型的新同源品系小鼠作为受体时，在成年期长期给予无细胞抗原，使用脾提取物可获得高达100%（根据抗原剂量）的永久耐受动物（尤其是雄性），而肝提取物则不能。这与肝提取物在各种非H-2不相容性中具有更高的耐受诱导效率形成对比。在供体-受体组合颠倒的情况下，脾提取物和肝提取物均未产生阳性结果。对永久耐受动物的免疫状态进行了分析，结果表明，其骨髓可赋予致死性照射的同基因宿主正常反应性，而特异性同种异体移植耐受可通过脾或淋巴结细胞过继转移给亚致死性照射的受体。得出的结论是，在本系统中，同种异体移植耐受状态是由外周淋巴细胞的免疫活性过程维持的。
