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脉孢菌中转录与翻译的时间分离。

Temporal separation of transcription and translation in Neurospora.

作者信息

Turner J R, Terry K, Matchett W H

出版信息

J Bacteriol. 1970 Aug;103(2):370-4. doi: 10.1128/jb.103.2.370-374.1970.

Abstract

Cells of Neurospora accumulated capacity for synthesis of kynureninase in the presence of cycloheximide and a suitable inducer. The capacity was expressed only when the cells were returned to a medium which allowed net synthesis of protein to resume. Subsequent expression did not require synthesis of ribonucleic acid (i.e., was not inhibited by actinomycin D) or the presence of inducer. The relative level of capacity, as judged by the initial rate of appearance of enzyme, increased linearly for 70 min in cells incubated in the presence of inducer plus antibiotic.

摘要

在环己酰亚胺和合适的诱导剂存在的情况下,粗糙脉孢菌的细胞积累了犬尿氨酸酶的合成能力。只有当细胞回到允许蛋白质净合成恢复的培养基中时,这种能力才会表达。随后的表达不需要核糖核酸的合成(即不受放线菌素D的抑制),也不需要诱导剂的存在。根据酶出现的初始速率判断,在诱导剂加抗生素存在下培养的细胞中,能力的相对水平在70分钟内呈线性增加。

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本文引用的文献

1
RNA SYNTHESIS OF YEAST IN THE PRESENCE OF CYCLOHEXIMIDE.在环己酰亚胺存在下酵母的RNA合成
Biochem Biophys Res Commun. 1965 Jan 18;18:297-301. doi: 10.1016/0006-291x(65)90757-6.
3
THE THEORY OF INTER-ALLELIC COMPLEMENTATION.等位基因间互补理论
J Mol Biol. 1964 Jan;8:161-5. doi: 10.1016/s0022-2836(64)80156-x.
7
Induction of kynureninase in Neurospora.脉孢菌中犬尿氨酸酶的诱导
J Bacteriol. 1970 Aug;103(2):364-9. doi: 10.1128/jb.103.2.364-369.1970.
8
Level of tryptophan messenger RNA in Escherichia coli.大肠杆菌中色氨酸信使核糖核酸的水平
J Mol Biol. 1968 Oct 28;37(2):289-302. doi: 10.1016/0022-2836(68)90268-4.

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