Wigglesworth V B
J Cell Sci. 1975 Dec;19(3):425-37. doi: 10.1242/jcs.19.3.425.
Tissues fixed in osmium tetroxide or in combined osmium and glutaraldehyde (Hinde), embedded in Spurr's medium, cut at 0-5-I mum and mounted in Farrants' gum medium containing ethyl gallate, show good staining of lipid-contaning structures (droplets of triglyceride, membranes, mitochondria, etc.) in the light microscope. Such preparations show moderate contrast in the electron microscope without further staining. But a specific increase in contrast in lipid-rich structures is obtained by partition of the tissues, before embedding, in 70% ethanol saturated with the monoterpene hydrocarbon myrcene, with or without the addition of 0-I % ethyl gallate, followed by osmium tetroxide. This method will visualize both saturated and unsaturated lipids, including waxes.
用四氧化锇或锇与戊二醛混合液（欣德法）固定、包埋于斯珀树脂介质中、切成0.5 - 1微米厚并置于含没食子酸乙酯的法伦特树胶介质中的组织，在光学显微镜下显示出含脂结构（甘油三酯滴、膜、线粒体等）的良好染色。此类标本在电子显微镜下无需进一步染色就有适度的反差。但是，在包埋前，将组织置于用单萜烃月桂烯饱和的70%乙醇中（添加或不添加0.1%没食子酸乙酯），然后用四氧化锇处理，可使富含脂质的结构的反差显著增加。该方法能使包括蜡质在内的饱和及不饱和脂质都显现出来。
