Tam Y K, Axelson J E
J Chromatogr. 1979 Feb 11;170(1):157-63. doi: 10.1016/s0021-9673(00)84247-8.
A procedure is described for determining 4-amino-5-chloro-2-methoxy-N-(2-ethylaminoethyl)benzamide, a metabolite formed by de-ethylation of metochlopramide, in urine from rats. The sampe is extracted (at pH approximately 13) with chloroform and, after treatment with heptafluorobutyric anhydride, derivatives of the extracted compounds are analyzed by gas-liquid chromatography, with electron-capture detection and diazepam as internal standard. The drug and its metabolite are separated, and the latter can be determined in the range 0.4 to 1.85 microgram/ml in the sample. The behaviour of the metabolic during chemical-ionization and electron-impact mass spectrometry is discussed.
本文描述了一种测定大鼠尿液中4-氨基-5-氯-2-甲氧基-N-(2-乙氨基乙基)苯甲酰胺的方法，该物质是甲氧氯普胺脱乙基形成的代谢产物。样品（在pH约为13时）用氯仿萃取，经七氟丁酸酐处理后，萃取化合物的衍生物通过气相色谱分析，采用电子捕获检测，以地西泮作为内标。药物及其代谢产物可分离，样品中代谢产物的测定范围为0.4至1.85微克/毫升。文中还讨论了该代谢产物在化学电离和电子轰击质谱中的行为。
