Jones R D, Blanton C D, Bowen J M
Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Vet Res Commun. 1993;17(5):387-96. doi: 10.1007/BF01839389.
The urinary metabolites of metoclopramide (4-amino-5-chloro-N-[2-diethylaminoethyl]-2-methoxybenzamide) were identified in cows. The drug was administered intravenously, voided urine was collected, and individual urine extracts were analysed by gas chromatography-mass spectrometry and high-performance liquid chromatography-photodiode array detection. The parent compound and one major metabolite (4-amino-5-chloro-N-[2-(ethylamino)ethyl]-2-methoxybenzamide) were common to all individuals. In addition to the parent and major metabolite, a second, minor metabolite was identified in two cows as 4-amino-5-chloro-N-[2-(diethylamino)ethyl]-2-hydroxybenzamide. The identity of the minor metabolite was confirmed by comparison with a standard synthesized by a new method. Metabolite identification and characterization in food animal species allows the design of safety and environmental impact studies and relative metabolite ratios between dose treatment groups.
在奶牛体内鉴定出了胃复安（4-氨基-5-氯-N-[2-二乙氨基乙基]-2-甲氧基苯甲酰胺）的尿液代谢物。通过静脉注射给予该药物，收集排尿后的尿液，并采用气相色谱-质谱联用仪和高效液相色谱-光电二极管阵列检测法对各个尿液提取物进行分析。母体化合物和一种主要代谢物（4-氨基-5-氯-N-[2-(乙氨基)乙基]-2-甲氧基苯甲酰胺）在所有个体中均有发现。除了母体和主要代谢物外，在两头奶牛中鉴定出了第二种次要代谢物，即4-氨基-5-氯-N-[2-(二乙氨基)乙基]-2-羟基苯甲酰胺。通过与采用新方法合成的标准品进行比较，证实了次要代谢物的身份。对食用动物物种中的代谢物进行鉴定和表征，有助于设计安全性和环境影响研究以及剂量处理组之间的相对代谢物比率。
