Goodchild B, Smith J N
Biochem J. 1970 May;117(5):1005-9. doi: 10.1042/bj1171005.
Electrophoresis in a sucrose gradient at pH values between 5 and 8 separated housefly DDT [1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane] dehydrochlorinase into two major fractions. GSH S-aryltransferase under similar conditions migrated as a single peak of activity. Separation of housefly homogenates or partially purified enzyme preparations by electrofocusing in a natural pH gradient also showed the presence of multiple forms of DDT dehydrochlorinase.
在pH值为5至8的蔗糖梯度中进行电泳,将家蝇滴滴涕[1,1,1 - 三氯 - 2,2 - 双 -(对氯苯基)乙烷]脱氯化氢酶分离成两个主要部分。在类似条件下,谷胱甘肽S - 芳基转移酶以单一活性峰迁移。通过在天然pH梯度中进行电聚焦分离家蝇匀浆或部分纯化的酶制剂,也显示出存在多种形式的滴滴涕脱氯化氢酶。