通过电聚焦和电泳从谷胱甘肽S-芳基转移酶中分离家蝇1,1,1-三氯-2,2-双（对氯苯基）乙烷（滴滴涕）脱氯化氢酶的多种形式
The separation of multiple forms of housefly 1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane (DDT) dehydrochlorinase from glutathione S-aryltransferase by electrofocusing and electrophoresis.
作者信息
Goodchild B, Smith J N
出版信息
Biochem J. 1970 May;117(5):1005-9. doi: 10.1042/bj1171005.
Abstract
Electrophoresis in a sucrose gradient at pH values between 5 and 8 separated housefly DDT [1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane] dehydrochlorinase into two major fractions. GSH S-aryltransferase under similar conditions migrated as a single peak of activity. Separation of housefly homogenates or partially purified enzyme preparations by electrofocusing in a natural pH gradient also showed the presence of multiple forms of DDT dehydrochlorinase.
摘要
在pH值为5至8的蔗糖梯度中进行电泳，将家蝇滴滴涕[1,1,1 - 三氯 - 2,2 - 双 -（对氯苯基）乙烷]脱氯化氢酶分离成两个主要部分。在类似条件下，谷胱甘肽S - 芳基转移酶以单一活性峰迁移。通过在天然pH梯度中进行电聚焦分离家蝇匀浆或部分纯化的酶制剂，也显示出存在多种形式的滴滴涕脱氯化氢酶。
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引用本文的文献
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2
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本文引用的文献
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Biochem J. 1961 Jun;79(3):516-24. doi: 10.1042/bj0790516.
2
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3
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Biochem J. 1953 Aug;55(1):170-1. doi: 10.1042/bj0550170.
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On the heterogeneity of beef heart cytochrome c. II. Some physico-chemical properties of the main subfractions (Cy I-Cy 3).论牛心细胞色素c的异质性。II. 主要亚组分（Cy I - Cy 3）的一些物理化学性质
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