一些脊椎动物和昆虫谷胱甘肽转移酶以甲基对硫磷（二甲基对硝基苯基硫代磷酸酯）、1-氯-2,4-二硝基苯和反式巴豆酰-N-乙酰半胱氨酸为底物时的交叉特异性。
Cross specificity in some vertebrate and insect glutathione-transferases with methyl parathion (dimethyl p-nitrophenyl phosphorothionate), 1-chloro-2,4-dinitro-benzene and s-crotonyl-N-acetylcysteamine as substrates.
作者信息
Clark A G, Smith J N, Speir T W
出版信息
Biochem J. 1973 Nov;135(3):385-92. doi: 10.1042/bj1350385.
Abstract
- Enzymes catalysing the reaction between GSH and methylparathion (dimethyl p-nitrophenyl phosphorothionate), 1-chloro-2,4-dinitrobenzene and S-crotonyl-N-acetylcysteamine were separated by (NH(4))(2)SO(4) precipitation from homogenates of sheep, rat and mouse livers and from homogenates of cockroaches, houseflies and grass grubs. 2. Electrofocusing of the preparations from each of these species separated a number of zones, each of which catalysed the reaction of GSH with all three substrates. 3. Ion-exchange chromatography on CM-cellulose also separated a number of fractions in which activity towards the three substrates coincided. 4. In both separation methods patterns of the activities were consistent with the presence in all species of several GSH transferases each having a degree of cross specificity towards the three substrates.
摘要
- 通过硫酸铵沉淀法，从绵羊、大鼠和小鼠肝脏匀浆以及蟑螂、家蝇和蛴螬匀浆中分离出催化谷胱甘肽（GSH）与甲基对硫磷（二甲基对硝基苯基硫代磷酸酯）、1-氯-2,4-二硝基苯和S-巴豆酰基-N-乙酰半胱氨酸之间反应的酶。2. 对这些物种各自的制剂进行等电聚焦，分离出多个区域，每个区域都催化GSH与所有三种底物的反应。3. 在CM-纤维素上进行离子交换色谱也分离出多个部分，其中对三种底物的活性一致。4. 在两种分离方法中，活性模式与所有物种中存在几种谷胱甘肽转移酶一致，每种酶对三种底物都具有一定程度的交叉特异性。
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Cross specificity in some vertebrate and insect glutathione-transferases with methyl parathion (dimethyl p-nitrophenyl phosphorothionate), 1-chloro-2,4-dinitro-benzene and s-crotonyl-N-acetylcysteamine as substrates.一些脊椎动物和昆虫谷胱甘肽转移酶以甲基对硫磷（二甲基对硝基苯基硫代磷酸酯）、1-氯-2,4-二硝基苯和反式巴豆酰-N-乙酰半胱氨酸为底物时的交叉特异性。
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本文引用的文献
1
An enzyme from rat liver catalysing conjugations with glutathione.一种来自大鼠肝脏的可催化与谷胱甘肽结合反应的酶。
Biochem J. 1961 Jun;79(3):516-24. doi: 10.1042/bj0790516.
2
THE CHEMISTRY AND METABOLISM OF RECENTLY ISOLATED AMINO ACIDS.新分离氨基酸的化学性质与代谢
Annu Rev Biochem. 1964;33:173-204. doi: 10.1146/annurev.bi.33.070164.001133.
3
BIOSYNTHESIS OF ISOVALTHINE PRECURSOR IN LIVER HOMOGENATES.肝脏匀浆中异缬氨酸前体的生物合成
Biochim Biophys Acta. 1963 Nov 15;78:553-5. doi: 10.1016/0006-3002(63)90924-7.
4
Metabolic incorporation of L-valine-[14C] into S-(2-carboxypropyl) glutathione and S-(2-carboxypropyl) cysteine in garlic.大蒜中L-缬氨酸-[14C]向S-(2-羧丙基)谷胱甘肽和S-(2-羧丙基)半胱氨酸的代谢掺入。
Chem Pharm Bull (Tokyo). 1962 Apr;10:328-31. doi: 10.1248/cpb.10.328.
5
Acidic peptides of the lens. 8. S-(alpha beta-dicarboxyethyl) glutathione.晶状体的酸性肽。8. S-（αβ-二羧乙基）谷胱甘肽。
Biochem J. 1963 Feb;86(2):226-31. doi: 10.1042/bj0860226.
6
Metabolism of benzene hexachloride by resistant houseflies Musca domestica.抗性家蝇（Musca domestica）对六氯环己烷的代谢
Nature. 1959 Apr 4;183(4666):983-4. doi: 10.1038/183983b0.
7
Isoelectric focusing and separation of the subcomponents of lactoperoxidase.乳过氧化物酶亚组分的等电聚焦及分离
Acta Chem Scand. 1967;21(1):271-8. doi: 10.3891/acta.chem.scand.21-0271.
8
Enzyme-catalysed conjugations of glutathione with unsaturated compounds.谷胱甘肽与不饱和化合物的酶催化共轭反应。
Biochem J. 1967 Jul;104(1):95-102. doi: 10.1042/bj1040095.
9
Species differences in the inhibition of glutathione S-aryltransferase by phthaleins and dicarboxylic acids.酚酞和二元羧酸对谷胱甘肽S-芳基转移酶抑制作用的种属差异。
Biochem J. 1967 Apr;103(1):49-54. doi: 10.1042/bj1030049.
10
Nature of a soluble, glutathione-dependent enzyme system active in cleavage of methyl parathion to desmethyl parathion.一种可溶的、依赖谷胱甘肽的酶系统的性质，该系统在将甲基对硫磷裂解为去甲基对硫磷的过程中具有活性。
J Econ Entomol. 1966 Dec;59(6):1338-46. doi: 10.1093/jee/59.6.1338.
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