Ellen R P, Leung W L, Fillery E D, Grove D A
Infect Immun. 1979 Nov;26(2):427-34. doi: 10.1128/iai.26.2.427-434.1979.
Rapid agglutination of Actinomyces viscosus and Actinomyces naeslundii cells by D-mannose solutions was observed during studies of their attachment to mammalian cells in vitro. The specificity of the agglutination reaction was studied by slide agglutination tests and by measuring the rate of decrease in optical density of bacterial phosphate buffer suspensions caused by the setting of bacterial aggregates. Actinomyces cells were agglutinated by protein-containing mannose solutions of several chemical suppliers. Solutions of sugars other than D-mannose and solutions of mannitol and mannan all failed to agglutinate A. viscsus and A. naeslundii. "Mannose-enhanced" agglutination was impaired by boiling or autoclaving the mannose but was not affected by heating the bacteria, the presence of chloramphenicol, running the assay in the cold, or incorporating any of several commercially purchased sugars in the reaction mixture. During these hapten inhibition experiments, only 6-deoxy-L-talcose-containing extracts of an A. viscosus strain retarded the rate of mannose-enhanced agglutination. Protein-containing fractions of D-mannose mother liquors also agglutinated cells of A. viscosus and A. naeslundii. Other species of oral gram-positive rods were not agglutinated by mannose solutions. Together the data indicate that plant seed-derived D-mannose contains a protein-associated agglutinin for A. viscosus and A. naeslundii which may function via a "lectin-like" selective affinity for the unique cell wall sugar 6-deoxy-L-talose.
在对粘性放线菌和内氏放线菌体外黏附哺乳动物细胞的研究过程中，观察到D-甘露糖溶液可使这些细菌细胞快速凝集。通过玻片凝集试验以及测量细菌聚集体沉降导致的细菌磷酸盐缓冲液悬浮液光密度降低速率，对凝集反应的特异性进行了研究。来自几家化学供应商的含蛋白质的甘露糖溶液均可使放线菌细胞凝集。除D-甘露糖之外的其他糖类溶液以及甘露醇和甘露聚糖溶液均不能使粘性放线菌和内氏放线菌凝集。将甘露糖煮沸或高压灭菌会削弱“甘露糖增强型”凝集反应，但加热细菌、存在氯霉素、在低温下进行试验或在反应混合物中加入几种商业购买的糖类中的任何一种对此均无影响。在这些半抗原抑制实验中，只有一种粘性放线菌菌株的含6-脱氧-L-塔罗糖提取物可减缓甘露糖增强型凝集反应的速率。D-甘露糖母液中的含蛋白质部分也可使粘性放线菌和内氏放线菌细胞凝集。其他口腔革兰氏阳性杆菌则不会被甘露糖溶液凝集。这些数据共同表明，植物种子来源的D-甘露糖含有一种与蛋白质相关的凝集素，可作用于粘性放线菌和内氏放线菌，其作用可能是通过对独特的细胞壁糖类6-脱氧-L-塔罗糖具有“类凝集素样”选择性亲和力来实现的。
