McGillen J, Phair J
Infect Immun. 1979 Nov;26(2):542-6. doi: 10.1128/iai.26.2.542-546.1979.
The effect of orally administered glucocorticoids on polymorphonuclear leukocyte adherence was studied by using the nylon fiber adherence assay. Inhibition of adherence 4 h after administration of the agent was confirmed, and in addition, augmentation of adherence was noted 24 h after ingestion of prednisone. Crossover studies revealed inhibition and augmentation to be cell associated as well as plasma mediated. Suspension of washed, dextran-sedimented polymorphonuclear leukocytes, harvested 4 h after prednisone ingestion in base-line or 24-h plasma failed to reverse inhibition of adherence. Adherence of 24-h polymorphonuclear leukocytes was augmented when suspended in all test plasmas. Plasma-mediated effects were demonstrated by inhibition of base-line adherence of polymorphonuclear leukocytes suspended in 4-h plasma and augmentation of adherence of cells in 24-h plasma. Plasma-mediated effects were reversible by washing.
通过尼龙纤维黏附试验研究了口服糖皮质激素对多形核白细胞黏附的影响。证实给药后4小时黏附受到抑制，此外，摄入泼尼松后24小时观察到黏附增强。交叉研究表明，抑制和增强与细胞及血浆介导有关。将泼尼松摄入后4小时采集的洗涤过的、经右旋糖酐沉降的多形核白细胞悬浮于基线或24小时血浆中，未能逆转黏附抑制。当悬浮于所有测试血浆中时，24小时多形核白细胞的黏附增强。多形核白细胞悬浮于4小时血浆中的基线黏附受到抑制，而细胞在24小时血浆中的黏附增强，证明了血浆介导的效应。血浆介导的效应可通过洗涤逆转。
